A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels
文献类型：期刊论文
| 作者 | Jiang, Guo-Qiang; Yao, Xue-Feng; Liu, Chun-Ming
|
| 刊名 | PLANT MOLECULAR BIOLOGY REPORTER
 |
| 出版日期 | 2013 |
| 卷号 | 31期号:6页码:1325-1335 |
| 关键词 | CELI SNP InDel Agarose gel electrophoresis Capillary electrophoresis |
| ISSN号 | 0735-9640 |
| DOI | 10.1007/s11105-013-0606-z |
| 文献子类 | Article |
| 英文摘要 | Modern genetic analyses rely on efficient genotyping of single-nucleotide polymorphisms (SNP) or insertion/deletion length polymorphisms (InDel) in genomes. Methods available to genotype these polymorphisms include sequencing, cleaved amplified polymorphic sequence, high-resolution DNA melting, and microarray analyses, which are all rather tedious or expensive to set up for daily use. Here, we report a simplified label-free CELI endonuclease (CELI)-based protocol that enables us to detect both SNPs and InDels for fragment lengths between 500 and 6 kb. PCR-amplified target DNA fragments were annealed, cleaved by CELI, and analyzed either cost-effectively by agarose gel electrophoresis or automatically by capillary electrophoresis. The optimal amplification sizes, potential blind ends, and the maximum pooling capacities were examined for both electrophoresis protocols. We believe that the CELI-based genotyping protocol can be used in the detection of mutations, marker-assisted breeding, map-based cloning, and genome-wide association studies. |
| 学科主题 | Biochemical Research Methods ; Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1572-9818 |
| WOS关键词 | INDUCED MUTATIONS ; MELTING ANALYSIS ; RICE ; DISCOVERY ; POLYMORPHISMS ; SEQUENCE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000326804400014 |
| 出版者 | SPRINGER |
| 资助机构 | National Transgenic Research Projects ; EU CEDROME project |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28148]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.[Jiang, Guo-Qiang 4.Yao, Xue-Feng |
| 推荐引用方式 GB/T 7714 | Jiang, Guo-Qiang,Yao, Xue-Feng,Liu, Chun-Ming. A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels[J]. PLANT MOLECULAR BIOLOGY REPORTER,2013,31(6):1325-1335. |
| APA | Jiang, Guo-Qiang,Yao, Xue-Feng,&Liu, Chun-Ming.(2013).A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels.PLANT MOLECULAR BIOLOGY REPORTER,31(6),1325-1335. |
| MLA | Jiang, Guo-Qiang,et al."A Simple CELI Endonuclease-Based Protocol for Genotyping both SNPs and InDels".PLANT MOLECULAR BIOLOGY REPORTER 31.6(2013):1325-1335. |
入库方式： OAI收割
来源：植物研究所
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