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Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization

文献类型：期刊论文

作者Wang, Qinli; Zhao, Yuanyuan; Luo, Wangxi6; Li, Ruili; He, Qihua2; Fang, Xiaohong6; De Michele, Roberto3,5; Ast, Cindy3; von Wiren, Nicolaus4; Lin, Jinxing
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2013
卷号110期号:32页码:13204-13209
关键词VA-TIRFM FCS
ISSN号0027-8424
DOI10.1073/pnas.1301160110
文献子类Article
英文摘要Ammonium is a preferred source of nitrogen for plants but is toxic at high levels. Plant ammonium transporters (AMTs) play an essential role in NH4+ uptake, but the mechanism by which AMTs are regulated remains unclear. To study how AMTs are regulated in the presence of ammonium, we used variable-angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy for single-particle fluorescence imaging of EGFP-tagged AMT1;3 on the plasma membrane of Arabidopsis root cells at various ammonium levels. We demonstrated that AMT1;3-EGFP dynamically appeared and disappeared on the plasma membrane as moving fluorescent spots in low oligomeric states under N-deprived and N-sufficient conditions. Under external high-ammonium stress, however, AMT1;3-EGFPs were found to amass into clusters, which were then internalized into the cytoplasm. A similar phenomenon also occurred in the glutamine synthetase mutant gln1;2 background. Single-particle analysis of AMT1;3-EGFPs in the clathrin heavy chain 2 mutant (chc2 mutant) and Flotllin1 artificial microRNA (Flot1 amiRNA) backgrounds, together with chemical inhibitor treatments, demonstrated that the endocytosis of AMT1;3 clusters induced by high-ammonium stress could occur mainly through clathrin-mediated endocytic pathways, but the contribution of microdomain-associated endocytic pathway cannot be excluded in the internalization. Our results revealed that the clustering and endocytosis of AMT1;3 provides an effective mechanism by which plant cells can avoid accumulation of toxic levels of ammonium by eliminating active AMT1;3 from the plasma membrane.
学科主题Multidisciplinary Sciences
出版地WASHINGTON
WOS关键词PLASMA-MEMBRANE ; DYNAMICS ; ENDOCYTOSIS ; ORGANIZATION ; MICROSCOPY ; MECHANISM ; STEROLS ; PROTEIN ; GROWTH
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000322771100079
出版者NATL ACAD SCIENCES
资助机构973 Program(National Basic Research Program of China) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of Sciences) ; National Nature Science Foundation of China Project Grant
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28150]  
专题中科院植物分子生理学重点实验室
作者单位1.Leibniz Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany
2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China
4.Natl Res Council Italy, Inst Plant Genet, I-90123 Palermo, Italy
5.Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA
6.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Qinli,Zhao, Yuanyuan,Luo, Wangxi,et al. Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(32):13204-13209.
APA Wang, Qinli.,Zhao, Yuanyuan.,Luo, Wangxi.,Li, Ruili.,He, Qihua.,...&Lin, Jinxing.(2013).Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(32),13204-13209.
MLA Wang, Qinli,et al."Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.32(2013):13204-13209.

入库方式： OAI收割

来源：植物研究所

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