Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms
文献类型：期刊论文
| 作者 | Liu, Shujun1; Sun, Yonghua1; Du, Xiaoqiu; Xu, Qijiang3; Wu, Feng; Meng, Zheng |
| 刊名 | ANNALS OF BOTANY
 |
| 出版日期 | 2013 |
| 卷号 | 112期号:7页码:1239-1251 |
| 关键词 | Floral homeotic B function Hedyosmum orientale Chloranthaceae C-terminal region HoAP3 HoPI homodimerization APETALA3 PISTILLATA |
| ISSN号 | 0305-7364 |
| DOI | 10.1093/aob/mct182 |
| 文献子类 | Article |
| 英文摘要 | According to the floral ABC model, B-function genes appear to play a key role in the origin and diversification of the perianth during the evolution of angiosperms. The basal angiosperm Hedyosmum orientale (Chloranthaceae) has unisexual inflorescences associated with a seemingly primitive reproductive morphology and a reduced perianth structure in female flowers. The aim of this study was to investigate the nature of the perianth and the evolutionary state of the B-function programme in this species. A series of experiments were conducted to characterize B-gene homologues isolated from H. orientale, including scanning electron microscopy to observe the development of floral organs, phylogenetic analysis to reconstruct gene evolutionary history, reverse transcriptionPCR, quantitative real-time PCR and in situ hybridization to identify gene expression patterns, the yeast two-hybrid assay to explore protein dimerization affinities, and transgenic analyses in Arabidopsis thaliana to determine activities of the encoded proteins. The expression of HoAP3 genes was restricted to stamens, whereas HoPI genes were broadly expressed in all floral organs. HoAP3 was able to partially restore the stamen but not petal identity in Arabidopsis ap3-3 mutants. In contrast, HoPI could rescue aspects of both stamen and petal development in Arabidopsis pi-1 mutants. When the complete C-terminal sequence of HoPI was deleted, however, no or weak transgenic phenotypes were observed and homodimerization capability was completely abolished. The results suggest that Hedyosmum AP3-like genes have an ancestral function in specifying male reproductive organs, and that the activity of the encoded PI-like proteins is highly conserved between Hedyosmum and Arabidopsis. Moreover, there is evidence that the C-terminal region is important for the function of HoPI. Our findings indicate that the development of the proposed perianth in Hedyosmum does not rely on the B homeotic function. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1095-8290 |
| WOS关键词 | MADS-BOX GENE ; LILY LILIUM-LONGIFLORUM ; ORGAN IDENTITY ; NEO-FUNCTIONALIZATION ; ECTOPIC EXPRESSION ; ANTIRRHINUM-MAJUS ; CONTROLLING PETAL ; ABC MODEL ; PI MOTIF ; ARABIDOPSIS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000326289900011 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Nature Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28159]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Northeast Forestry Univ, Dept Bot, Haerbin 150040, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Shujun,Sun, Yonghua,Du, Xiaoqiu,et al. Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms[J]. ANNALS OF BOTANY,2013,112(7):1239-1251. |
| APA | Liu, Shujun,Sun, Yonghua,Du, Xiaoqiu,Xu, Qijiang,Wu, Feng,&Meng, Zheng.(2013).Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms.ANNALS OF BOTANY,112(7),1239-1251. |
| MLA | Liu, Shujun,et al."Analysis of the APETALA3- and PISTILLATA-like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms".ANNALS OF BOTANY 112.7(2013):1239-1251. |
入库方式： OAI收割
来源：植物研究所
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