GUS activity for miR165a/166b, REV, and WUS/CLV3 in in vitro direct Arabidopsis thaliana shoot regeneration
文献类型：期刊论文
| 作者 | Liu, Zhenhua; Xin, Wei1; Ji, Dandan; Wang, Long; Li, Juan; Xiang, Fengning |
| 刊名 | PROTOPLASMA
 |
| 出版日期 | 2013 |
| 卷号 | 250期号:5页码:1213-1218 |
| 关键词 | miR165/166 REV WUS/CLV3 GUS activity Direct shoot regeneration |
| ISSN号 | 0033-183X |
| DOI | 10.1007/s00709-013-0503-1 |
| 文献子类 | Article |
| 英文摘要 | In Arabidopsis thaliana, the process of shoot regeneration in vitro requires the presence of specific miRNAs. We describe here the beta-glucuronidase (GUS) expression domains for miR165a/166b, REV, and WUS/CLV3 during direct shoot regeneration. Increased GUS activity of miR166b and REV were first detected within the shoot apical meristem of explants, while no pmiR165a::GUS activity appeared there. The zone of pWUS::GUS activity covered the inner sides of developing protuberances, while that of pCLV3::GUS was more restricted. Once the primary shoot had emerged from the protuberance, pREV::GUS activity was turned on throughout the protuberance. pmiR165a::GUS activity was detected in a small number of protuberance surface cells, while pWUS::GUS activity was restricted to within a few cells beneath the protuberance surface. After the differentiation of leaf-like structures, GUS activity for miR165a and miR166b appeared largely on their abaxial surface, while pWUS::GUS activity was concentrated at the apex of the primary shoot, and no pCLV3::GUS activity was detectable. Following the formation of secondary shoots, pmiR165a::GUS activity was detected on their abaxial surface. GUS activity for miR166b, REV, and WUS/CLV3 were concentrated in the stem apical meristem. The observations suggested that each member of this set of genes might play a distinct role in both primary and secondary shoot regeneration. |
| 学科主题 | Plant Sciences ; Cell Biology |
| 出版地 | WIEN |
| 电子版国际标准刊号 | 1615-6102 |
| WOS关键词 | STEM-CELL FATE ; EXPRESSION PATTERNS ; APICAL MERISTEM ; GENE ; WUSCHEL ; ROOT |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000325132800023 |
| 出版者 | SPRINGER WIEN |
| 资助机构 | Chinese National Special Science Research Program ; Key Projects in the National Science and Technology Pillar Program in the 12th Five-Year Plan of China ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; International technology cooperation project of Shandong Province of China ; Chinese Natural Education Ministry Doctor Station Foundation Fellowship |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28163]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Bot, Ctr Signal Transduct & Metabol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhenhua,Xin, Wei,Ji, Dandan,et al. GUS activity for miR165a/166b, REV, and WUS/CLV3 in in vitro direct Arabidopsis thaliana shoot regeneration[J]. PROTOPLASMA,2013,250(5):1213-1218. |
| APA | Liu, Zhenhua,Xin, Wei,Ji, Dandan,Wang, Long,Li, Juan,&Xiang, Fengning.(2013).GUS activity for miR165a/166b, REV, and WUS/CLV3 in in vitro direct Arabidopsis thaliana shoot regeneration.PROTOPLASMA,250(5),1213-1218. |
| MLA | Liu, Zhenhua,et al."GUS activity for miR165a/166b, REV, and WUS/CLV3 in in vitro direct Arabidopsis thaliana shoot regeneration".PROTOPLASMA 250.5(2013):1213-1218. |
入库方式： OAI收割
来源：植物研究所
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