Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes
文献类型：期刊论文
| 作者 | Liu, Chang-Cai; Zhu, Hang-Yong; Dong, Xiu-Mei; Ning, De-Li; Wang, Hong-Xia; Li, Wei-Hua; Yang, Chuan-Ping; Wang, Bai-Chen |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:3 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0058681 |
| 文献子类 | Article |
| 英文摘要 | Background: The N-terminal protein processing mechanism (NPM) including N-terminal Met excision (NME) and N-terminal acetylation (N-alpha-acetylation) represents a common protein co-translational process of some eukaryotes. However, this NPM occurred in woody plants yet remains unknown. Methodology/Principal Findings: To reveal the NPM in poplar, we investigated the N-alpha-acetylation status of poplar proteins during dormancy by combining tandem mass spectrometry with TiO2 enrichment of acetylated peptides. We identified 58 N-terminally acetylated (N-alpha-acetylated) proteins. Most proteins (47, >81%) are subjected to N-alpha-acetylation following the N-terminal removal of Met, indicating that N-alpha-acetylation and NME represent a common NPM of poplar proteins. Furthermore, we confirm that poplar shares the analogous NME and N-alpha-acetylation (NPM) to other eukaryotes according to analysis of N-terminal features of these acetylated proteins combined with genome-wide identification of the involving methionine aminopeptidases (MAPs) and N-terminal acetyltransferase (Nat) enzymes in poplar. The N-alpha-acetylated reactions and the involving enzymes of these poplar proteins are also identified based on those of yeast and human, as well as the subcellular location information of these poplar proteins. Conclusions/Significance: This study represents the first extensive investigation of N-alpha-acetylation events in woody plants, the results of which will provide useful resources for future unraveling the regulatory mechanisms of N-alpha-acetylation of proteins in poplar. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | HIGHLY SELECTIVE ENRICHMENT ; ALPHA-ACETYLTRANSFERASE ; PHOSPHORYLATED PEPTIDES ; METHIONINE EXCISION ; YEAST ; DYNAMICS ; GENOME ; IMPACT ; STROMA |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000316251100079 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Basic Research Priorities Program(National Basic Research Program of China) ; State Key Program of the National Natural Science of China(National Natural Science Foundation of China (NSFC)) ; Northeast Forestry University |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27795]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.[Liu, Chang-Cai 2.[Wang, Hong-Xia 3.Natl Ctr Biomed Anal, Inst Basic Med Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 5.Bur Garden & Pk, Qitaihe, Heilongjiang, Peoples R China 6.Hubei Nanxing Gen Chem Factory, Lab Chem Def & Microscale Anal, Zhijiang, Hubei, Peoples R China 7.Ning, De-Li 8.Dong, Xiu-Mei 9.Zhu, Hang-Yong 10.Northeast Forestry Univ, State Key Lab Forest Genet & Tree Breeding, Harbin, Heilongjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Chang-Cai,Zhu, Hang-Yong,Dong, Xiu-Mei,et al. Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes[J]. PLOS ONE,2013,8(3). |
| APA | Liu, Chang-Cai.,Zhu, Hang-Yong.,Dong, Xiu-Mei.,Ning, De-Li.,Wang, Hong-Xia.,...&Wang, Bai-Chen.(2013).Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes.PLOS ONE,8(3). |
| MLA | Liu, Chang-Cai,et al."Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes".PLOS ONE 8.3(2013). |
入库方式： OAI收割
来源：植物研究所
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