Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica
文献类型：期刊论文
| 作者 | Li, Minchun2; Zhao, Mingming2; Wu, Hanying; Wu, Wang2; Xu, Yinong |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 55期号:6页码:490-503 |
| 关键词 | Diacylglycerol acyltransferase1 Tetraena mongolica triacylglycerol |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/jipb.12046 |
| 文献子类 | Article |
| 英文摘要 | Two cDNAs encoding putative type 1 acyl-CoA: diacylglycerol acyltransferases (DGAT1, EC 2.3.1.20), were cloned from Tetraena mongolica, an extreme xerophyte with high oil content in the stems. The 1 488-bp and 1 485-bp of the open reading frame (ORF) of the two cDNAs, designated as TmDGAT1a and TmDGAT1b, were both predicted to encode proteins of 495 and 494 amino acids, respectively. Southern blot analysis revealed that TmDGAT1a and TmDGAT1b both had low copy numbers in the T. mongolica genome. In addition to ubiquitous expression with different intensity in different tissues, including stems, leaves and roots, TmDGAT1a and TmDGAT1b, were found to be strongly induced by high salinity, drought and osmotic stress, resulting in a remarkable increase of triacylglycerol (TAG) accumulation in T. mongolica plantlets. TmDGAT1a and TmDGAT1b activities were confirmed in the yeast H1246 quadruple mutant (DGA1, LRO1, ARE1, ARE2) by restoring DGAT activity of the mutant host to produce TAG. Overexpression of TmDGAT1a and TmDGAT1b in soybean hairy roots as well as in T. mongolica calli both resulted in an increase in oil content (ranging from 37% to 108%), accompanied by altered fatty acid profiles. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1744-7909 |
| WOS关键词 | BRASSICA-NAPUS ; OIL CONTENT ; TRIACYLGLYCEROL SYNTHESIS ; ENDOPLASMIC-RETICULUM ; LIPID-SYNTHESIS ; FATTY-ACID ; EXPRESSION ; ENZYME ; BIOSYNTHESIS ; TOBACCO |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000320276600002 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China(National Basic Research Program of China) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27989]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Minchun,Zhao, Mingming,Wu, Hanying,et al. Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2013,55(6):490-503. |
| APA | Li, Minchun,Zhao, Mingming,Wu, Hanying,Wu, Wang,&Xu, Yinong.(2013).Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,55(6),490-503. |
| MLA | Li, Minchun,et al."Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 55.6(2013):490-503. |
入库方式： OAI收割
来源：植物研究所
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