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Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress

文献类型：期刊论文

作者Zhong, Linlin2; Zhou, Wen2; Wang, Haijun2; Ding, Shunhua; Lu, Qingtao; Wen, Xiaogang; Peng, Lianwei; Zhang, Lixin3; Lu, Congming3
刊名PLANT CELL
出版日期2013
卷号25期号:8页码:2925-2943
ISSN号1040-4651
DOI10.1105/tpc.113.111229
文献子类Article
英文摘要Compared with small heat shock proteins (sHSPs) in other organisms, those in plants are the most abundant and diverse. However, the molecular mechanisms by which sHSPs are involved in cell protection remain unknown. Here, we characterized the role of HSP21, a plastid nucleoid-localized sHSP, in chloroplast development under heat stress. We show that an Arabidopsis thaliana knockout mutant of HSP21 had an ivory phenotype under heat stress. Quantitative real-time RT-PCR, run-on transcription, RNA gel blot, and polysome association analyses demonstrated that HSP21 is involved in plastid-encoded RNA polymerase (PEP)-dependent transcription. We found that the plastid nucleoid protein pTAC5 was an HSP21 target. pTAC5 has a C4-type zinc finger similar to that of Escherichia coli DnaJ and zinc-dependent disulfide isomerase activity. Reduction of pTAC5 expression by RNA interference led to similar phenotypic effects as observed in hsp21. HSP21 and pTAC5 formed a complex that was associated mainly with the PEP complex. HSP21 and pTAC5 were associated with the PEP complex not only during transcription initiation, but also during elongation and termination. Our results suggest that HSP21 and pTAC5 are required for chloroplast development under heat stress by maintaining PEP function.
学科主题Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
出版地ROCKVILLE
WOS关键词ENCODED RNA-POLYMERASE ; DISULFIDE OXIDOREDUCTASE ACTIVITIES ; DNA-BINDING PROTEIN ; GENE-EXPRESSION ; PHOTOSYSTEM-II ; OXIDATIVE STRESS ; HIGHER-PLANTS ; CHLOROPHYLL BIOSYNTHESIS ; QUATERNARY DYNAMICS ; CHAPERONE FUNCTION
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000324920500016
出版者AMER SOC PLANT BIOLOGISTS
资助机构State Key Basic Research and Development Plan of China(National Basic Research Program of China) ; Solar Energy Initiative of the Chinese Academy of Sciences(Chinese Academy of Sciences)
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28049]  
专题中科院光生物学重点实验室
作者单位1.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Zhong, Linlin,Zhou, Wen,Wang, Haijun,et al. Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress[J]. PLANT CELL,2013,25(8):2925-2943.
APA Zhong, Linlin.,Zhou, Wen.,Wang, Haijun.,Ding, Shunhua.,Lu, Qingtao.,...&Lu, Congming.(2013).Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress.PLANT CELL,25(8),2925-2943.
MLA Zhong, Linlin,et al."Chloroplast Small Heat Shock Protein HSP21 Interacts with Plastid Nucleoid Protein pTAC5 and Is Essential for Chloroplast Development in Arabidopsis under Heat Stress".PLANT CELL 25.8(2013):2925-2943.

入库方式： OAI收割

来源：植物研究所

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