Expression of the CMV-CP Gene in Synechocystis 6803 Affects Cyanobacterial Photosynthesis
文献类型：期刊论文
| 作者 | Wang, Chun-Mei; Shi, Ding-Ji; Zhu, Shui-Fang; Yie, Yin1; Tien, Po1; Wei, Ning-Sheng |
| 刊名 | JOURNAL OF PHYTOPATHOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 161期号:4页码:263-270 |
| 关键词 | Cucumber mosaic virus coat protein photosynthesis transgenic cyanobacterium Synechocystis sp PCC 6803 |
| ISSN号 | 0931-1785 |
| DOI | 10.1111/jph.12049 |
| 文献子类 | Article |
| 英文摘要 | Previous work has shown that the presence of excess coat protein (CP) of cucumber mosaic virus (CMV) in the chloroplasts was related with mosaic symptoms. However, whether these mosaic symptoms are directly induced by the interaction between CP and chloroplasts is unknown. To directly demonstrate the interaction between CP and the chloroplast, Synechocystis sp. PCC 6803 was used as the chloroplast model. The cDNA encoding the CMV-CP was cloned in a cyanobacterial shuttle vector (pKT-CP) and transferred to Synechocystis sp. PCC 6803. The CP was expressed in the cyanobacterium with the psbA promoter. The expression of CMV-CP hindered the growth of transgenic cyanobacterium cells and decreased its photosynthetic rate and the PS II activity. The transgenic cells showed increased fluorescence (F) from the phycobilisome terminal emitters and increased fluorescence (F) from PS II. The absorption spectra at room temperature showed the Chl and the phycocyanin absorption peak of the mutant strain significantly decreased. These results showed that CP may directly affect the cyanobacterium cells and decreased its photosynthesis, especially the PS II activity. These data might provide new evidence for mosaic symptoms being directly induced by the interaction between CP and chloroplasts. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1439-0434 |
| WOS关键词 | TOBACCO-MOSAIC-VIRUS ; COAT PROTEIN GENE ; PHOTOSYSTEM-II ; THYLAKOID MEMBRANES ; ELECTRON-TRANSPORT ; MOVEMENT PROTEIN ; STRAIN-Y ; LEAVES ; CHLOROPLASTS ; CUCUMBER |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000315970000007 |
| 出版者 | WILEY |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28066]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Inspect & Quarantine, Inst Anim & Plant Quarantine, Beijing 100029, Peoples R China 2.Beijing Univ Chinese Med, Beijing 100102, Peoples R China 3.Northwest Agr & Forestry Univ, Coll Plant Protect, Yangling 712100, Shanxi, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 5.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chun-Mei,Shi, Ding-Ji,Zhu, Shui-Fang,et al. Expression of the CMV-CP Gene in Synechocystis 6803 Affects Cyanobacterial Photosynthesis[J]. JOURNAL OF PHYTOPATHOLOGY,2013,161(4):263-270. |
| APA | Wang, Chun-Mei,Shi, Ding-Ji,Zhu, Shui-Fang,Yie, Yin,Tien, Po,&Wei, Ning-Sheng.(2013).Expression of the CMV-CP Gene in Synechocystis 6803 Affects Cyanobacterial Photosynthesis.JOURNAL OF PHYTOPATHOLOGY,161(4),263-270. |
| MLA | Wang, Chun-Mei,et al."Expression of the CMV-CP Gene in Synechocystis 6803 Affects Cyanobacterial Photosynthesis".JOURNAL OF PHYTOPATHOLOGY 161.4(2013):263-270. |
入库方式： OAI收割
来源：植物研究所
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