Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance
文献类型：期刊论文
| 作者 | Wang, Xuchu3; Chang, Lili3; Wang, Baichen2; Wang, Dan; Li, Pinghua3; Wang, Limin3; Yi, Xiaoping; Huang, Qixing; Peng, Ming; Guo, Anping3 |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2013 |
| 卷号 | 12期号:8页码:2174-2195 |
| ISSN号 | 1535-9476 |
| DOI | 10.1074/mcp.M112.022475 |
| 文献子类 | Article |
| 英文摘要 | Thellungiella halophila, a close relative of Arabidopsis, is a model halophyte used to study plant salt tolerance. The proteomic/physiological/transcriptomic analyses of Thellungiella plant leaves subjected to different salinity levels, reported herein, indicate an extraordinary ability of Thellungiella to adapt to large concentrations of exogenous saline by compartmentalizing Na+ into cell vacuoles and accumulating proline and soluble sugars as organic osmolytes. Salinity stress stimulated the accumulation of starch in chloroplasts, which resulted in a greatly increased content of starch and total sugars in leaves. Comparative proteomics of Thellungiella leaves identified 209 salt-responsive proteins. Among these, the sequences of 108 proteins were strongly homologous to Arabidopsis protein sequences, and 30 had previously been identified as Thellungiella proteins. Functional classification of these proteins into 16 categories indicated that the majority are involved in carbohydrate metabolism, followed by those involved in energy production and conversion, and then those involved in the transport of inorganic ions. Pathway analysis revealed that most of the proteins are involved in starch and sucrose metabolism, carbon fixation, photosynthesis, and glycolysis. Of these processes, the most affected were starch and sucrose metabolism, which might be pivotal for salt tolerance. The gene expression patterns of the 209 salt-responsive proteins revealed through hierarchical clustering of microarray data and the expression patterns of 29 Thellungiella genes evaluated via quantitative RT-PCR were similar to those deduced via proteomic analysis, which underscored the possibility that starch and sucrose metabolism might play pivotal roles in determining the salt tolerance ability of Thellungiella. Our observations enabled us to propose a schematic representation of the systematic salt-tolerance phenotype in Thellungiella and suggested that the increased accumulation of starch, soluble sugars, and proline, as well as subcellular compartmentalization of sodium, might collectively denote important mechanisms for halophyte salt tolerance. |
| 学科主题 | Biochemical Research Methods |
| 出版地 | BETHESDA |
| 电子版国际标准刊号 | 1535-9484 |
| WOS关键词 | ARABIDOPSIS-THALIANA ; GENE-EXPRESSION ; NA+ TRANSPORT ; STRESS ; GROWTH ; ACCUMULATION ; PROLINE ; MODEL ; NACL ; OVEREXPRESSION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000328839100012 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 资助机构 | National Basic Research Program (973) of China(National Basic Research Program of China) ; National Natural Science Foundation(National Natural Science Foundation of China (NSFC)) ; Program for the Top Young Talents in Chinese Academy of Tropical Agricultural Sciences |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28097]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 2.Hainan Univ, Haikou 570228, Hainan, Peoples R China 3.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Trop Crops, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xuchu,Chang, Lili,Wang, Baichen,et al. Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance[J]. MOLECULAR & CELLULAR PROTEOMICS,2013,12(8):2174-2195. |
| APA | Wang, Xuchu.,Chang, Lili.,Wang, Baichen.,Wang, Dan.,Li, Pinghua.,...&Guo, Anping.(2013).Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance.MOLECULAR & CELLULAR PROTEOMICS,12(8),2174-2195. |
| MLA | Wang, Xuchu,et al."Comparative Proteomics of Thellungiella halophila Leaves from Plants Subjected to Salinity Reveals the Importance of Chloroplastic Starch and Soluble Sugars in Halophyte Salt Tolerance".MOLECULAR & CELLULAR PROTEOMICS 12.8(2013):2174-2195. |
入库方式： OAI收割
来源：植物研究所
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