Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources
文献类型：期刊论文
| 作者 | He Jun; Han Guangye2; Chen Daiwen |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2013 |
| 卷号 | 89页码:191-201 |
| 关键词 | Trichoderma reesei Proteome Carbon source Enzyme Regulation |
| ISSN号 | 1874-3919 |
| DOI | 10.1016/j.jprot.2013.06.014 |
| 文献子类 | Article |
| 英文摘要 | Trichoderma reesei is the main industrial producer of lignocellulolytic enzymes, and the secretory behavior of this fungus strongly depends on the carbon sources. To gain insights into how the T. reesei adapts to various carbons and regulates enzyme production, the extra-and intracellular proteomes of T. reesei grown in defined medium with lactose or xylose as the carbon source were investigated. Results indicated that the composition of extracellular proteome differed considerably depending on the carbons. The main cellobiohydrolases, i.e. Cel7a/Cel6 were the most abundant cellulolytic enzymes identified in both media, and found to be more abundant in lactose-grown culture. As compared to lactose, xylose can serve as a potent inducer of xylanolytic enzymes. Interestingly, most identified intracellular proteins are involved in carbon metabolism. Enzymes involved in utilization of xylose, such as n-xylose reductase (Xyl1p) and n-xylose dehydrogenase (Xyl2p), were present at elevated levels in the culture growing on xylose but only in minor amounts in the lactose culture. However, lactose induction significantly activated the expression of key enzymes involved in glycolysis pathway and citrate cycle. Importantly, the protein Xyl1p which participates both in the lactose and the xylose catabolism was verified as a potential regulator for cellulase formation in T. reesei. Biological significance This study not only gives an overview of the ubiquitous cellular changes induced by the two conventional carbon substrates, but offers the framework for understanding the mechanisms behind the carbon-dependent induction of extracellular enzymes in T. reesei. Moreover, this study provides a potential target (Xyl1p) that could be tentatively used for metabolic engineering of T. reesei for cost-effective cellulase production. (C) 2013 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemical Research Methods |
| 出版地 | AMSTERDAM |
| 电子版国际标准刊号 | 1876-7737 |
| WOS关键词 | CELLULASE GENE-EXPRESSION ; HYPOCREA-JECORINA ; SACCHAROMYCES-CEREVISIAE ; BETA-GALACTOSIDASE ; ASPERGILLUS-NIGER ; INDUCTION ; XYLOSE ; XYLANASE ; HEMICELLULASES ; TRANSFORMATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000323867200016 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Special Fund of Chongqing Key Laboratory (CSTC)(Natural Science Foundation Project of CQ CSTC) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28108]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | He Jun,Han Guangye,Chen Daiwen. Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources[J]. JOURNAL OF PROTEOMICS,2013,89:191-201. |
| APA | He Jun,Han Guangye,&Chen Daiwen.(2013).Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources.JOURNAL OF PROTEOMICS,89,191-201. |
| MLA | He Jun,et al."Insights into enzyme secretion by filamentous fungi: Comparative proteome analysis of Trichoderma reesei grown on different carbon sources".JOURNAL OF PROTEOMICS 89(2013):191-201. |
入库方式： OAI收割
来源：植物研究所
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