Enhanced H-2 photoproduction by down-regulation of ferredoxin-NADP(+) reductase (FNR) in the green alga Chlamydomonas reinhardtii
文献类型：期刊论文
| 作者 | Sun, Yongle1; Chen, Mei; Yang, Haomeng; Zhang, Jin1; Kuang, Tingyun; Huang, Fang
|
| 刊名 | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
 |
| 出版日期 | 2013 |
| 卷号 | 38期号:36页码:16029-16037 |
| 关键词 | Chlamydomonas H-2 photoproduction FNR RNAi Sulfur depletion |
| ISSN号 | 0360-3199 |
| DOI | 10.1016/j.ijhydene.2013.10.011 |
| 文献子类 | Article |
| 英文摘要 | Renewable H-2 photoproduction by green algae such as Chlamydomonas reinhardtii is a promising system for solar fuels. However, large-scale application of the system has lagged virtually due to lack of high H-2-producing strains. We previously identified ferredoxin-NADP(+) reductase (FNR) among the 105 proteins differentially expressed in Chlamydomonas during sulfur-deprived H-2 photoproduction. In this work, we used an RNA interference (RNAi) approach to generate Chlamydomonas mutant strains with reduced levels of FNR. We found that fnr-RNAi strains exhibited higher rates of H-2 photoproduction (2.5-fold) than wild type under sulfur-deprived condition. To elucidate the basis for this increase, we analyzed the physiological characteristics of the fnr-RNAi strains under such condition. Major changes, due to the down-regulation of FNR, included the lower rates of photosynthetic O-2 evolution (44%), greater reduction of Rubisco amounts (60%) and higher rates of starch degradation (140%). These may result in an earlier onset of anaerobiosis and increased electron supply to the hydrogenases in the mutant strains. The results provide new information of FNR in regulating H-2 metabolism as well as potential strains for further improvement of the organism toward application in solar-powered systems. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. |
| 学科主题 | Chemistry, Physical ; Electrochemistry ; Energy & Fuels |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1879-3487 |
| WOS关键词 | HYDROGEN-PRODUCTION ; PROTEIN ; ANTENNA ; STRAINS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000327904500020 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; Chinese Academy of Sciences(Chinese Academy of Sciences) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28129]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yongle,Chen, Mei,Yang, Haomeng,et al. Enhanced H-2 photoproduction by down-regulation of ferredoxin-NADP(+) reductase (FNR) in the green alga Chlamydomonas reinhardtii[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2013,38(36):16029-16037. |
| APA | Sun, Yongle,Chen, Mei,Yang, Haomeng,Zhang, Jin,Kuang, Tingyun,&Huang, Fang.(2013).Enhanced H-2 photoproduction by down-regulation of ferredoxin-NADP(+) reductase (FNR) in the green alga Chlamydomonas reinhardtii.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,38(36),16029-16037. |
| MLA | Sun, Yongle,et al."Enhanced H-2 photoproduction by down-regulation of ferredoxin-NADP(+) reductase (FNR) in the green alga Chlamydomonas reinhardtii".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 38.36(2013):16029-16037. |
入库方式： OAI收割
来源：植物研究所
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