A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex
文献类型：期刊论文
| 作者 | Qin, Xiaochun; Zhu, Jiajia1; Wang, Wenda3; Ding, Xiang1; Wang, Kebin; Fang, Yan1; Kuang, Tingyun |
| 刊名 | JOURNAL OF RAMAN SPECTROSCOPY
 |
| 出版日期 | 2013 |
| 卷号 | 44期号:8页码:1111-1119 |
| 关键词 | carotenoid carotene Photosystem I SERS SERRS |
| ISSN号 | 0377-0486 |
| DOI | 10.1002/jrs.4333 |
| 文献子类 | Article |
| 英文摘要 | In plants, Photosystem I (PSI) is composed of a core complex and a membrane-associated antenna complex light-harvesting complex I that captures light and funnels its energy to the core complex. To obtain Raman structural information on -carotenes embedded in the PSI core complex, a sandwich' system of roughened silver slice: target protein complexes: single silver nanoparticles was fabricated for Surface-Enhanced Resonance Raman Scattering (SERRS) measurements. This study provided a method to overcome spectral irreproducibility, which is the main drawback of Surface-Enhanced Raman Scattering/SERRS-based studies. The Raman spectra of -carotenes embedded in the PSI core complex can be obtained at very low sample concentrations (1-5 mu g Chl/ml) and high signal/noise ratios. The -carotenes in the spinach PSI core complex were predominantly all-trans configuration. The membrane protein-mediated adsorption of silver nanoparticles induced the uniform distribution of a large number of single nanoparticles, which contributed to achieving highly reproducible SERRS spectra. This study is the first to apply single silver nanoparticle-based SERRS analysis in membrane proteins. Copyright (c) 2013 John Wiley & Sons, Ltd. |
| 学科主题 | Spectroscopy |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1097-4555 |
| WOS关键词 | PLANT PHOTOSYSTEM-I ; REACTION CENTERS ; SILVER ; SPECTROSCOPY ; BACTERIAL ; NANOPARTICLES ; MEMBRANES ; SPECTRA ; DISCRIMINATION ; FLUORESCENCE |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000323180700007 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China (973 Program)(National Basic Research Program of China) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28178]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Capital Normal Univ, Beijing Key Lab Nanophoton & Nanostruct, Beijing 100048, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Xiaochun,Zhu, Jiajia,Wang, Wenda,et al. A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex[J]. JOURNAL OF RAMAN SPECTROSCOPY,2013,44(8):1111-1119. |
| APA | Qin, Xiaochun.,Zhu, Jiajia.,Wang, Wenda.,Ding, Xiang.,Wang, Kebin.,...&Kuang, Tingyun.(2013).A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex.JOURNAL OF RAMAN SPECTROSCOPY,44(8),1111-1119. |
| MLA | Qin, Xiaochun,et al."A stable sandwich' system of Surface-Enhanced Resonance Raman Scattering for the analysis of -carotenes in a photosynthetic pigment-protein complex".JOURNAL OF RAMAN SPECTROSCOPY 44.8(2013):1111-1119. |
入库方式： OAI收割
来源：植物研究所
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