Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress
文献类型：期刊论文
| 作者 | Zhao, Lei2; Chen, Mei; Cheng, Dongmei2; Yang, Haomeng; Sun, Yongle2; Zhou, Heyi2; Huang, Fang
|
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 55期号:11页码:1054-1068 |
| 关键词 | Chlamydomonas high light MSRB oxidative stress sulfur deprivation |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/jipb.12104 |
| 文献子类 | Article |
| 英文摘要 | The genome of unicellular green alga Chlamydomonas reinhardtii contains four genes encoding B-type methionine sulfoxide reductases, MSRB1.1, MSRB1.2, MSRB2.1, and MSRB2.2, with functions largely unknown. To understand the cell defense system mediated by the methionine sulfoxide reductases in Chlamydomonas, we analyzed expression and physiological roles of the MSRBs under different abiotic stress conditions using immunoblotting and quantitative polymerase chain reaction (PCR) analyses. We showed that the MSRB2.2 protein was accumulated in cells treated with high light (1,300 mu E/m(2) per s), whereas MSRB1.1 was accumulated in the cells under 1mmol/L H2O2 treatment or sulfur depletion. We observed that the cells with the MSRB2.2 knockdown and overexpression displayed increased and decreased sensitivity to high light, respectively, based on in situ chlorophyll a fluorescence measures. We also observed that the cells with the MSRB1.1 knockdown and overexpression displayed decreased and increased tolerance to sulfur-depletion and oxidative stresses, respectively, based on growth and H-2-producing performance. The physiological implications revealed from the experimental data highlight the importance of MSRB2.2 and MSRB1.1 in protecting Chlamydomonas cells against adverse conditions such as high-light, sulfur-depletion, and oxidative stresses. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1744-7909 |
| WOS关键词 | MACROALGA ULVA-FASCIATA ; PHOTOSYSTEM-II ; PHOTOOXIDATIVE STRESS ; ARABIDOPSIS ; REINHARDTII ; EXPRESSION ; EVOLUTION ; MAMMALS ; MSRA ; PHOTOSYNTHESIS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000328148600006 |
| 出版者 | WILEY-BLACKWELL |
| 资助机构 | Ministry of Science and Technology of China(Ministry of Science and Technology, China) ; Chinese Ministry of Agriculture(Ministry of Agriculture & Rural Affairs) ; Chinese Academy of Sciences(Chinese Academy of Sciences) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28186]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Lei,Chen, Mei,Cheng, Dongmei,et al. Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2013,55(11):1054-1068. |
| APA | Zhao, Lei.,Chen, Mei.,Cheng, Dongmei.,Yang, Haomeng.,Sun, Yongle.,...&Huang, Fang.(2013).Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,55(11),1054-1068. |
| MLA | Zhao, Lei,et al."Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 55.11(2013):1054-1068. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。