Metabolic Engineering of the Phenylpropanoid Pathway Enhances the Antioxidant Capacity of Saussurea involucrata
文献类型：期刊论文
| 作者 | Qiu, Jian1; Gao, Fenghua; Shen, Guoan; Li, Chonghui; Han, Xiaoyan; Zhao, Qiao3; Zhao, Dexiu; Hua, Xuejun; Pang, Yongzhen |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:8 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0070665 |
| 文献子类 | Article |
| 英文摘要 | The rare wild species of snow lotus Saussurea involucrata is a commonly used medicinal herb with great pharmacological value for human health, resulting from its uniquely high level of phenylpropanoid compound production. To gain information on the phenylpropanid biosynthetic pathway genes in this critically important medicinal plant, global transcriptome sequencing was performed. It revealed that the phenylpropanoid pathway genes were well represented in S. involucrata. In addition, we introduced two key phenylpropanoid pathway inducing transcription factors (PAP1 and Lc) into this medicinal plant. Transgenic S. involucrata co-expressing PAP1 and Lc exhibited purple pigments due to a massive accumulation of anthocyanins. The over-expression of PAP1 and Lc largely activated most of the phenylpropanoid pathway genes, and increased accumulation of several phenylpropanoid compounds significantly, including chlorogenic acid, syringin, cyanrine and rutin. Both ABTS (2,2'-azinobis-3-ethylbenzotiazo-line-6-sulfonic acid) and FRAP (ferric reducing antioxidant power) assays revealed that the antioxidant capacity of transgenic S. involucrata lines was greatly enhanced over controls. In addition to providing a deeper understanding of the molecular basis of phenylpropanoid metabolism, our results potentially enable an alternation of bioactive compound production in S. involucrata through metabolic engineering. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | MYB TRANSCRIPTION FACTOR ; ARABIDOPSIS-THALIANA ; MAIZE LC ; BIOSYNTHESIS ; PROANTHOCYANIDINS ; ANTHOCYANINS ; EXPRESSION ; GENE ; BIOCHEMISTRY ; FLAVONOIDS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000323221500021 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27799]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing, Peoples R China 2.Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73402 USA 3.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Biol & Genet Resources Rubber Tree, Danzhou, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Jian,Gao, Fenghua,Shen, Guoan,et al. Metabolic Engineering of the Phenylpropanoid Pathway Enhances the Antioxidant Capacity of Saussurea involucrata[J]. PLOS ONE,2013,8(8). |
| APA | Qiu, Jian.,Gao, Fenghua.,Shen, Guoan.,Li, Chonghui.,Han, Xiaoyan.,...&Pang, Yongzhen.(2013).Metabolic Engineering of the Phenylpropanoid Pathway Enhances the Antioxidant Capacity of Saussurea involucrata.PLOS ONE,8(8). |
| MLA | Qiu, Jian,et al."Metabolic Engineering of the Phenylpropanoid Pathway Enhances the Antioxidant Capacity of Saussurea involucrata".PLOS ONE 8.8(2013). |
入库方式： OAI收割
来源：植物研究所
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