Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress
文献类型：期刊论文
| 作者 | Xin, Haiping2; Zhu, Wei1; Wang, Lina1; Xiang, Yue1; Fang, Linchuan1; Li, Jitao1; Sun, Xiaoming1; Wang, Nian2; Londo, Jason P.; Li, Shaohua2
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| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:3 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0058740 |
| 文献子类 | Article |
| 英文摘要 | Grape is one of the most important fruit crops worldwide. The suitable geographical locations and productivity of grapes are largely limited by temperature. Vitis amurensis is a wild grapevine species with remarkable cold-tolerance, exceeding that of Vitis vinifera, the dominant cultivated species of grapevine. However, the molecular mechanisms that contribute to the enhanced freezing tolerance of V. amurensis remain unknown. Here we used deep sequencing data from restriction endonuclease-generated cDNA fragments to evaluate the whole genome wide modification of transcriptome of V. amurensis under cold treatment. Vitis vinifera cv. Muscat of Hamburg was used as control to help investigate the distinctive features of V. amruensis in responding to cold stress. Approximately 9 million tags were sequenced from non-cold treatment (NCT) and cold treatment (CT) cDNA libraries in each species of grapevine sampled from shoot apices. Alignment of tags into V. vinifera cv. Pinot noir (PN40024) annotated genome identified over 15,000 transcripts in each library in V. amruensis and more than 16,000 in Muscat of Hamburg. Comparative analysis between NCT and CT libraries indicate that V. amurensis has fewer differential expressed genes (DEGs, 1314 transcripts) than Muscat of Hamburg (2307 transcripts) when exposed to cold stress. Common DEGs (408 transcripts) suggest that some genes provide fundamental roles during cold stress in grapes. The most robust DEGs (more than 20-fold change) also demonstrated significant differences between two kinds of grapevine, indicating that cold stress may trigger species specific pathways in V. amurensis. Functional categories of DEGs indicated that the proportion of up-regulated transcripts related to metabolism, transport, signal transduction and transcription were more abundant in V. amurensis. Several highly expressed transcripts that were found uniquely accumulated in V. amurensis are discussed in detail. This subset of unique candidate transcripts may contribute to the excellent cold-hardiness of V. amurensis. |
| 学科主题 | Multidisciplinary Sciences |
| 出版地 | SAN FRANCISCO |
| WOS关键词 | LOW-TEMPERATURE ; FREEZING TOLERANCE ; ABSCISIC-ACID ; GENE-EXPRESSION ; PROTEIN-KINASE ; ABIOTIC STRESS ; ARABIDOPSIS ; DROUGHT ; GRAPEVINE ; ACCLIMATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000316849200108 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 资助机构 | National Natural Science Foundation of China (NSFC)(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27802]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 3.Londo, Jason P.] ARS, USDA, Grape Genet Res Unit, Geneva, NY USA 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xin, Haiping,Zhu, Wei,Wang, Lina,et al. Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress[J]. PLOS ONE,2013,8(3). |
| APA | Xin, Haiping.,Zhu, Wei.,Wang, Lina.,Xiang, Yue.,Fang, Linchuan.,...&Li, Shaohua.(2013).Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress.PLOS ONE,8(3). |
| MLA | Xin, Haiping,et al."Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress".PLOS ONE 8.3(2013). |
入库方式： OAI收割
来源：植物研究所
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