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Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach

文献类型：期刊论文

作者Chen, Yanshan1; Xu, Wenzhong; Shen, Hongling1; Yan, Huili1; Xu, Wenxiu; He, Zhenyan; Ma, Mi
刊名ENVIRONMENTAL SCIENCE & TECHNOLOGY
出版日期2013
卷号47期号:16页码:9355-9362
ISSN号0013-936X
DOI10.1021/es4012096
文献子类Article
英文摘要Arsenic (As) pollution is a global problem, and the plant-based cleanup of contaminated soils, called phytoremediation, is therefore of great interest. Recently, transgenic approaches have been designed to develop As phytoremediation technologies. Here, we used a one-gene transgenic approach for As tolerance and accumulation in Arabidopsis thaliana. PvACR3, a key arsenite [As(III)] antiporter in the As hyperaccumulator fern Pteris vittata, was expressed in Arabidopsis, driven by the CaMV 35S promoter. In response to As treatment, PvACR3 transgenic plants showed greatly enhanced tolerance. PvACR3 transgenic seeds could even germinate and grow in the presence of 80 mu M As(III) or 1200 mu M arsenate [As(V)] treatments that were lethal to wildtype seeds. PvACR3 localizes to the plasma membrane in Arabidopsis and increases arsenite efflux into external medium in short-term experiments. Arsenic determination showed that PvACR3 substantially reduced As concentrations in roots and simultaneously increased shoot As under 150 mu M As(V). When cultivated in As(V)-containing soil (10 ppm As), transgenic plants accumulated approximately 7.5-fold more As in above-ground tissues than wild-type plants. This study provides important insights into the behavior of PvACR3 and the physiology of As metabolism in plants. Our work also provides a simple and practical PvACR3 transgenic approach for engineering As-tolerant and -hyperaccumulating plants for phytoremediation.
学科主题Engineering, Environmental ; Environmental Sciences
出版地WASHINGTON
电子版国际标准刊号1520-5851
WOS关键词PTERIS-VITTATA ; ACCUMULATION ; ARABIDOPSIS ; EFFLUX ; RICE ; FERN ; REDUCTION ; CADMIUM ; GROWTH
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000323471700045
出版者AMER CHEMICAL SOC
资助机构National Science Foundation Council of China(National Natural Science Foundation of China (NSFC))
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/27888]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Yanshan,Xu, Wenzhong,Shen, Hongling,et al. Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2013,47(16):9355-9362.
APA Chen, Yanshan.,Xu, Wenzhong.,Shen, Hongling.,Yan, Huili.,Xu, Wenxiu.,...&Ma, Mi.(2013).Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach.ENVIRONMENTAL SCIENCE & TECHNOLOGY,47(16),9355-9362.
MLA Chen, Yanshan,et al."Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach".ENVIRONMENTAL SCIENCE & TECHNOLOGY 47.16(2013):9355-9362.

入库方式： OAI收割

来源：植物研究所

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