Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli
文献类型：期刊论文
| 作者 | Kim, Yong-Goo; Sharmin, Shamima Akhtar; Alam, Iftekhar; Kim, Kyung-Hee; Kwon, Suk-Yoon; Sohn, Jung-Hoon; Kim, Sun-Hyung; Liu, Gongshe2; Lee, Byung-Hyun |
| 刊名 | GLOBAL CHANGE BIOLOGY BIOENERGY
 |
| 出版日期 | 2013 |
| 卷号 | 5期号:1页码:73-80 |
| ISSN号 | 1757-1693 |
| 关键词 | Agrobacterium bioenergy biomass Phragmites communis reed transgenic plant |
| DOI | 10.1111/j.1757-1707.2012.01200.x |
| 文献子类 | Article |
| 英文摘要 | Reed (Phragmites communis) is a potential bioenergy plant. We report on its first Agrobacterium-mediated transformation using mature seed-derived calli. The Agrobacterium strains LBA4404, EHA105, and GV3101, each harboring the binary vector pIG121Hm, were used to optimize T-DNA delivery into the reed genome. Bacterial strain, cocultivation period and acetosyringone concentration significantly influenced the T-DNA transfer. About 48% transient expression and 3.5% stable transformation were achieved when calli were infected with strain EHA105 for 10 min under 800 mbar negative pressure and cocultivated for 3 days in 200 mu m acetosyringone containing medium. Putative transformants were selected in 25 mg l-1 hygromycin B. PCR, and Southern blot analysis confirmed the presence of the transgenes and their stable integration. Independent transgenic lines contained one to three copies of the transgene. Transgene expression was validated by RT-PCR and GUS staining of stems and leaves. |
| 学科主题 | Agronomy ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 电子版国际标准刊号 | 1757-1707 |
| 出版地 | HOBOKEN |
| WOS关键词 | TALL FESCUE PLANTS ; SUSPENSION-CULTURES ; COMMON REED ; REGENERATION ; DIGESTIBILITY ; PROPAGATION ; VIRULENCE ; RESPONSES ; BARLEY |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000312131100009 |
| 资助机构 | KRIBB Research Initiative Program ; Next-Generation BioGreen 21 Program(Rural Development Administration (RDA), Republic of Korea) ; Rural Development Administration(Rural Development Administration (RDA), Republic of Korea) ; Cooperative Research Program for Agricultural Science & Technology Development, RDA, Republic of Korea ; Gyeongsang National University, Republic of Korea |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27923]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Univ Seoul, Dept Environm Hort, Seoul 130743, South Korea 3.Korea Res Inst Biosci & Biotechnol, Taejon 305806, South Korea 4.Gyeongsang Natl Univ, PMBBRC, IALS, Div Appl Life Sci,Program BK21, Jinju 660701, South Korea |
| 推荐引用方式 GB/T 7714 | Kim, Yong-Goo,Sharmin, Shamima Akhtar,Alam, Iftekhar,et al. Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli[J]. GLOBAL CHANGE BIOLOGY BIOENERGY,2013,5(1):73-80. |
| APA | Kim, Yong-Goo.,Sharmin, Shamima Akhtar.,Alam, Iftekhar.,Kim, Kyung-Hee.,Kwon, Suk-Yoon.,...&Lee, Byung-Hyun.(2013).Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli.GLOBAL CHANGE BIOLOGY BIOENERGY,5(1),73-80. |
| MLA | Kim, Yong-Goo,et al."Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli".GLOBAL CHANGE BIOLOGY BIOENERGY 5.1(2013):73-80. |
入库方式： OAI收割
来源：植物研究所
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