Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation
文献类型：期刊论文
| 作者 | Wen, Bin; Wang, Xiaofeng; Tan, Yunhong; Song, Songquan2 |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
 |
| 出版日期 | 2013 |
| 卷号 | 49期号:6页码:717-723 |
| 关键词 | Intermediate seeds Interspecific variation Intraspecies variation Cryotolerance Tropical seeds Germplasmconservation |
| ISSN号 | 1054-5476 |
| DOI | 10.1007/s11627-013-9562-4 |
| 文献子类 | Article |
| 英文摘要 | Several dehydration protocols were evaluated for their ability to cryopreserve intact seeds and excised embryonic axes of Mimusops elengi and Manilkara zapota (Sapotaceae). Both interspecific and intraspecific variations in cryotolerance were found. M. zapota embryonic axes were more tolerant of cryopreservation than those of M. elengi, and showed higher desiccation tolerance, higher post-thawing survival and development, and a much wider range of moisture contents for cryopreservation. Maximum development rates were 94% and 27% for M. zapota and M. elengi, respectively. Intact seeds of both species tolerated desiccation to low moisture levels, but were sensitive to liquid nitrogen exposure, and cryopreserved seeds failed to germinate. Assessment of developing embryos excised from cryopreserved seeds associated nonviability of cotyledons and plumules with germination failure. Other structures survived at variable rates; most hypocotyls and radicles (up to 76% and 98% for M. elengi and M. zapota, respectively) were viable. The different cryotolerance between hypocotyls and cotyledons is a critical cause for failure in cryopreservation, contributing to the difficulty in developing protocols for such intermediate oily seeds. |
| 学科主题 | Plant Sciences ; Cell Biology ; Developmental Biology |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1475-2689 |
| WOS关键词 | NEEM AZADIRACHTA-INDICA ; LIQUID-NITROGEN EXPOSURE ; COFFEA-ARABICA L. ; STORAGE BEHAVIOR ; OXIDATIVE STRESS ; ZYGOTIC EMBRYOS ; CITRUS ; WATER ; AXES ; TRIACYLGLYCEROLS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000328201700010 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Science and Technology Support Program |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27928]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Grp Seed Physiol & Biotechnol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Bin,Wang, Xiaofeng,Tan, Yunhong,et al. Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2013,49(6):717-723. |
| APA | Wen, Bin,Wang, Xiaofeng,Tan, Yunhong,&Song, Songquan.(2013).Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,49(6),717-723. |
| MLA | Wen, Bin,et al."Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 49.6(2013):717-723. |
入库方式： OAI收割
来源：植物研究所
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