A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis
文献类型：期刊论文
| 作者 | Li, Xiaoxia1; Gao, Qiong1; Liang, Ye1; Ma, Tian2; Cheng, Liqin; Qi, Dongmei; Liu, Hui; Xu, Xin2; Chen, Shuangyan; Liu, Gongshe
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| 刊名 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 64页码:52-59 |
| 关键词 | LcSAIN2 Novel salt-induced gene Salt tolerance Sheepgrass Arabidopsis |
| ISSN号 | 0981-9428 |
| DOI | 10.1016/j.plaphy.2012.12.014 |
| 文献子类 | Article |
| 英文摘要 | Salt stress affects plant growth and development, and limits the productivity of crops. Sheepgrass can grow well under various environmental and soil conditions and is a good wild resource in Triticeae. Using 454 high throughout sequencing technique, a large number of salt stress responsive genes have been picked out from sheepgrass. In this study, a novel salt-induced gene and its promoter were cloned and the gene was designated as LcSAIN2 (Leymus chinensis salt-induced 2). Bioinformatics analysis predicted that LcSAIN2 has one transmembrane helix and is localized in nucleus. Experiments of subcellular localization in tobacco leaf cells also indicated that it was mainly localized in nucleus. Several stress responsive elements were found in the promoter region of the LcSAIN2 gene. The expression analysis confirmed that LcSAIN2 was induced by salinity, PEG, ABA, and cold stresses, especially by high salinity. Overexpression of LcSAIN2 in Arabidopsis enhanced salt tolerance of transgenic plants by accumulating osmolytes, such as soluble sugars and free proline, and improving the expression levels of some stress-responsive transcription factors and key genes. Our results suggest that LcSAIN2 might play an important positive modulation role in salt stress tolerance and be a candidate gene utilized for enhancing stress tolerance in wheat and other crops. (C) 2013 Elsevier Masson SAS. All rights reserved. |
| 学科主题 | Plant Sciences |
| 出版地 | ISSY-LES-MOULINEAUX |
| 电子版国际标准刊号 | 1873-2690 |
| WOS关键词 | TRANSCRIPTIONAL REGULATORY NETWORKS ; FUNCTIONAL-ANALYSIS ; STRESS TOLERANCE ; DROUGHT ; EXPRESSION ; WHEAT ; COLD ; PROLINE ; PROTEIN ; OVEREXPRESSION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000315547100006 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National High Technology Research and Development Program of China (863)(National High Technology Research and Development Program of China) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences) ; Ningxia Agricultural Comprehensive Development Office |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/27977]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Ningxia Univ, Yinchuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiaoxia,Gao, Qiong,Liang, Ye,et al. A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2013,64:52-59. |
| APA | Li, Xiaoxia.,Gao, Qiong.,Liang, Ye.,Ma, Tian.,Cheng, Liqin.,...&Liu, Gongshe.(2013).A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis.PLANT PHYSIOLOGY AND BIOCHEMISTRY,64,52-59. |
| MLA | Li, Xiaoxia,et al."A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis".PLANT PHYSIOLOGY AND BIOCHEMISTRY 64(2013):52-59. |
入库方式： OAI收割
来源：植物研究所
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