Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins
文献类型：期刊论文
| 作者 | Wu, Ben-Hong; Niu, Ning; Li, Ji-Hu; Li, Shao-Hua
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| 刊名 | JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
 |
| 出版日期 | 2013 |
| 卷号 | 138期号:6页码:416-427 |
| 关键词 | anthocyanin berry composition crop load two-dimensional gel electrophoresis Vitis vinifera |
| ISSN号 | 0003-1062 |
| DOI | 10.21273/JASHS.138.6.416 |
| 文献子类 | Article |
| 英文摘要 | The most obvious effects of a low leaf:fruit (LF) ratio [two leaves for one cluster per shoot (LF2)] on grape (Vitis vinifera) berries are suppressed anthocyanin biosynthesis in the berry skin, decreased berry weight and soluble solids concentration, and increased titratable acidity. In this study, proteins isolated from berry skins grown under low and high LF ratio conditions, LF2 and LF12, respectively, were characterized by two-dimensional gel electrophoresis coupled to mass spectrometry. A survey of approximate to 600 to 700 spots from berry skin yielded 77 proteins with differential expression between LF12 and LF2 treatments. Of these, the 59 proteins that were identified consisted of 47 proteins that were down-regulated and 12 that were up-regulated under LF2 conditions compared with LF12 conditions. Most proteins involved in metabolism, energy, transcription, protein synthesis, binding function, signal transduction, and cell defense were down-regulated in LF2 berries, whereas two important enzymes of anthocyanin biosynthesis, chalcone synthase and dihydroflavonol reductase, were not detected. Only a few proteins (e.g., two heat shock proteins related to protein fate and nutrient reservoir storage protein) were found to be up-regulated in LF2 berries. This suggested that, with the exception of secondary metabolism, many proteomic events may have an effect on anthocyanin synthesis in the skins responding to LF. |
| 学科主题 | Horticulture |
| 出版地 | ALEXANDRIA |
| 电子版国际标准刊号 | 2327-9788 |
| WOS关键词 | VITIS-VINIFERA L ; FRUIT-QUALITY ; CROP LEVEL ; FLAVONOID BIOSYNTHESIS ; PRUNING SEVERITY ; SEASONAL-CHANGES ; TRAINING SYSTEM ; SHOOT DENSITY ; ORGANIC-ACIDS ; WINE QUALITY |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000333230400002 |
| 出版者 | AMER SOC HORTICULTURAL SCIENCE |
| 资助机构 | CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ministry of Agriculture(Gida Tarim Ve Hayvancilik Bakanligi) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28007]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Being Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Ben-Hong,Niu, Ning,Li, Ji-Hu,et al. Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins[J]. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,2013,138(6):416-427. |
| APA | Wu, Ben-Hong,Niu, Ning,Li, Ji-Hu,&Li, Shao-Hua.(2013).Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins.JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE,138(6),416-427. |
| MLA | Wu, Ben-Hong,et al."Leaf:fruit Ratio Affects the Proteomic Profile of Grape Berry Skins".JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE 138.6(2013):416-427. |
入库方式： OAI收割
来源：植物研究所
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