Jacalin domain in wheat jasmonate-regulated protein Ta-JA1 confers agglutinating activity and pathogen resistance
文献类型：期刊论文
| 作者 | Ma, Qing-Hu; Zhen, Wei-Bo; Liu, Yun-Chao |
| 刊名 | BIOCHIMIE
 |
| 出版日期 | 2013 |
| 卷号 | 95期号:2页码:359-365 |
| 关键词 | Chimeric protein Dirigent domain Jacalin-related lectin domain (JRL) Monocot jacalin-related lectins Triticum aestivum L |
| ISSN号 | 0300-9084 |
| DOI | 10.1016/j.biochi.2012.10.014 |
| 文献子类 | Article |
| 英文摘要 | Ta-JA1 is a jacalin-like lectin from wheat (Triticum aestivum) plants. To date, its homologs are only observed in the Gramineae family. Our previous experiments have demonstrated that Ta-JA1 contains a modular structure consisting of an N-terminal dirigent domain and a C-terminal jacalin-related lectin domain (JRL) and this protein exhibits a mannose-specific lectin activity. The over-expression of Ta-JA1 gene provides transgenic plants a broad-spectrum resistance to diseases. Here, we report the differential activities of the dirigent and JRL domains of Ta-JA1. In vitro assay showed that the recombinant JRL domain could effectively agglutinate rabbit erythrocytes and pathogen bacteria Pseudomonas syringe pv tabaci. These hemagglutination activities could be inhibited by mannose but not by galactose. In contrast, the recombinant dirigent domain did not show agglutination activity. Corresponding to these differentiations of activities, similar to full-length of Ta-JA1, the over-expression of JRL domain in transgenic plants also increased resistance to the infection of P. syringe. Unlike JRL, the over-expression of dirigent domain in transgenic plants led to alteration of the seedling sensitivity to salts. In addition, a d(N)/d(S) ratio analysis of Ta-JA1 and its related proteins showed that this protein family functionally limited to a few crop plants, such as maize, rice and wheat. (C) 2012 Elsevier Masson SAS. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology |
| 出版地 | PARIS |
| WOS关键词 | GLUCOSIDASE-AGGREGATING FACTOR ; LECTIN-LIKE GENE ; DISEASE RESISTANCE ; MAIZE ; EXPRESSION ; OVEREXPRESSION ; EVOLUTION ; SELECTION ; HOMOLOG ; SORGHUM |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000315614200027 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Natural Science Foundation of Beijing(Beijing Natural Science Foundation) ; Innovation Project of Chinese Academy of Sciences(Knowledge Innovation Program of the Chinese Academy of Sciences) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28025]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Zhen, Wei-Bo 2.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 3.[Ma, Qing-Hu |
| 推荐引用方式 GB/T 7714 | Ma, Qing-Hu,Zhen, Wei-Bo,Liu, Yun-Chao. Jacalin domain in wheat jasmonate-regulated protein Ta-JA1 confers agglutinating activity and pathogen resistance[J]. BIOCHIMIE,2013,95(2):359-365. |
| APA | Ma, Qing-Hu,Zhen, Wei-Bo,&Liu, Yun-Chao.(2013).Jacalin domain in wheat jasmonate-regulated protein Ta-JA1 confers agglutinating activity and pathogen resistance.BIOCHIMIE,95(2),359-365. |
| MLA | Ma, Qing-Hu,et al."Jacalin domain in wheat jasmonate-regulated protein Ta-JA1 confers agglutinating activity and pathogen resistance".BIOCHIMIE 95.2(2013):359-365. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。