中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage

文献类型:期刊论文

作者Zhu, Zhu1; Liu, Ruiling1; Li, Boqiang; Tian, Shiping
刊名FOOD CHEMISTRY
出版日期2013
卷号141期号:4页码:3323-3328
关键词Sugar metabolism Key genes Apple fruit Controlled atmosphere
ISSN号0308-8146
DOI10.1016/j.foodchem.2013.06.025
文献子类Article
英文摘要Sugars are essential contributors to fruit flavour. Controlled atmosphere (CA) storage has been proved to be beneficial for maintaining harvested fruit quality. To explore regulatory mechanism of sugar metabolism in fruit stored in CA condition, we cloned several genes, encoding key enzymes, involved in sugar metabolism in apple fruit, and analyzed sugar contents, along with gene expression and enzyme activities in fruits stored in air and CA. The results indicated that CA could maintain higher contents of sugars, including sucrose, fructose and glucose. Expression levels of key genes, such as sucrose synthase (SS), sucrose phosphate synthase (SPS), fructokinase (FK) and hexokinase (HK), were shown to be correlated with the corresponding enzyme activities. We found that activities of neutral invertase (NI), vacuolar invertase (VI), FM and HK were inhibited, but SPS activity was promoted in apple fruit stored in CA, suggesting that CA storage could enhance sucrose synthesis and delay hydrolysis of sucrose and hexose. These findings provided molecular evidence to explain why higher sugar levels in harvested fruit are maintained under CA storage. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
出版地OXFORD
电子版国际标准刊号1873-7072
WOS关键词SUCROSE-PHOSPHATE SYNTHASE ; OXYGEN ; PHYSIOLOGY ; INVERTASE ; QUALITY ; PLANTS ; O-2
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000324848000005
出版者ELSEVIER SCI LTD
资助机构National Basic Research Program of China (973 Program)(National Basic Research Program of China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28034]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Zhu, Zhu,Liu, Ruiling,Li, Boqiang,et al. Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage[J]. FOOD CHEMISTRY,2013,141(4):3323-3328.
APA Zhu, Zhu,Liu, Ruiling,Li, Boqiang,&Tian, Shiping.(2013).Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage.FOOD CHEMISTRY,141(4),3323-3328.
MLA Zhu, Zhu,et al."Characterisation of genes encoding key enzymes involved in sugar metabolism of apple fruit in controlled atmosphere storage".FOOD CHEMISTRY 141.4(2013):3323-3328.

入库方式: OAI收割

来源:植物研究所

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