Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.)
文献类型：期刊论文
| 作者 | Zhao, Zhuoya1; Chen, Yanshan1; Xu, Wenzhong; Ma, Mi |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 61期号:12页码:2964-2969 |
| 关键词 | surface plasmon resonance Cry1Ac cotton protein-based detection DNA-based detection |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/jf3050439 |
| 文献子类 | Article |
| 英文摘要 | The detection and identification of genetically modified (GM) plants are challenging issues that have arisen from the potential negative impacts of extensive cultivation of transgenic plants. The screening process is a long-term focus and needs specific detection strategies. Surface plasmon resonance (SPR) has been used to detect a variety of biomolecules including proteins and nucleic acids due to its ability to monitor specific intermolecular interactions. In the present study, two high-throughput, label-free, and specific methods based on SPR technology were developed to detect transgenic CrylAc cotton (Gossypium spp.) by separately targeting protein and DNA. In the protein-based detection system, monoclonal anti-Cry1Ac antibodies were immobilized on the surface of a CMS sensor chip. Conventional cotton samples were used to define the detection threshold. Transgenic cotton was easily identified within 5 min per sample. For the DNA-based model, a 25-mer biotinylated oligonucleotide probe was immobilized on an SA sensor chip. PCR products of CrylAc (230 bp) were used to investigate the reaction conditions. The sensitivity of the constructed sensor chip was identified at concentrations as low as 0.1 nM based on its complementary base pairing. |
| 学科主题 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 出版地 | WASHINGTON |
| WOS关键词 | GENETICALLY-MODIFIED ORGANISMS ; CHAIN-REACTION METHODS ; MODIFIED RICE ; BT COTTON ; MAIZE ; GENE ; CROPS ; ELISA ; PCR ; SEQUENCES |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000317031800009 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Transgenic Plant Special Fund ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28046]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Zhuoya,Chen, Yanshan,Xu, Wenzhong,et al. Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.)[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2013,61(12):2964-2969. |
| APA | Zhao, Zhuoya,Chen, Yanshan,Xu, Wenzhong,&Ma, Mi.(2013).Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.).JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,61(12),2964-2969. |
| MLA | Zhao, Zhuoya,et al."Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.)".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 61.12(2013):2964-2969. |
入库方式： OAI收割
来源：植物研究所
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