DETERMINATION OF LOTUS LEAF ALKALOIDS BY SOLID PHASE EXTRACTION COMBINED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY AND TANDEM MASS SPECTROMETRY DETECTION
文献类型：期刊论文
| 作者 | Chen, Sha2; Zhang, Haohao2; Liu, Yanling; Fang, Jinbao3; Li, Shaohua
|
| 刊名 | ANALYTICAL LETTERS
 |
| 出版日期 | 2013 |
| 卷号 | 46期号:18页码:2846-2859 |
| 关键词 | Alkaloids HPLC-MS Lotus germplasm PCA |
| ISSN号 | 0003-2719 |
| DOI | 10.1080/00032719.2013.816960 |
| 文献子类 | Article |
| 英文摘要 | Lotus (nelumbo) is a traditional Chinese medical herb, and alkaloids are responsible for its pharmaceutical activities. Therefore, a rapid, validated method to determine alkaloids in lotus leaves is useful for its quality control. The goal of this work was to develop and validate an analytical method for the identification and quantitation of the bioactive alkaloids of extracts from lotus leaf and classification of lotus germplasm based on alkaloid concentrations. Solid-phase extraction was used prior to high-performance liquid chromatography coupled with photodiode array detection at 272nm and tandem electrospray ionization triple quadrupole mass spectrometry for analysis. Nuciferine and O-nornuciferine were the dominant compounds in lotus used for seed production and some used for flower production. However, anonaine, roemerine, and N-nornuciferine were abundant only in some lotus flowers. All the lotuses were classified into four groups by principal component analysis of alkaloid content: 10 flower and three seed lotuses characterized by high nuciferine and O-nornuciferine content; 12 flower lotuses with high N-nornuciferine, roemerine, and anonaine content; two flower lotuses with a relatively high content of all five alkaloids; and cultivars with a low alkaloid content and without dominant alkaloids. |
| 学科主题 | Chemistry, Analytical |
| 出版地 | PHILADELPHIA |
| 电子版国际标准刊号 | 1532-236X |
| WOS关键词 | NELUMBO-NUCIFERA LEAVES ; TYROSINE-PHOSPHATASE ; GAERTN ; FLAVONOIDS ; INHIBITORS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000327180300005 |
| 出版者 | TAYLOR & FRANCIS INC |
| 资助机构 | Knowledge Innovation Project of Chinese Academy of Sciences(Knowledge Innovation Program of the Chinese Academy of Sciences) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28054]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Zhengzhou, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Sha,Zhang, Haohao,Liu, Yanling,et al. DETERMINATION OF LOTUS LEAF ALKALOIDS BY SOLID PHASE EXTRACTION COMBINED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY AND TANDEM MASS SPECTROMETRY DETECTION[J]. ANALYTICAL LETTERS,2013,46(18):2846-2859. |
| APA | Chen, Sha,Zhang, Haohao,Liu, Yanling,Fang, Jinbao,&Li, Shaohua.(2013).DETERMINATION OF LOTUS LEAF ALKALOIDS BY SOLID PHASE EXTRACTION COMBINED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY AND TANDEM MASS SPECTROMETRY DETECTION.ANALYTICAL LETTERS,46(18),2846-2859. |
| MLA | Chen, Sha,et al."DETERMINATION OF LOTUS LEAF ALKALOIDS BY SOLID PHASE EXTRACTION COMBINED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY AND TANDEM MASS SPECTROMETRY DETECTION".ANALYTICAL LETTERS 46.18(2013):2846-2859. |
入库方式： OAI收割
来源：植物研究所
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