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The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis

文献类型：期刊论文

作者Peng, Xianjun; Zhang, Lexin; Zhang, Lixing3; Liu, Zhujiang3; Cheng, Liqin; Yang, Ying1; Shen, Shihua; Chen, Shuangyan; Liu, Gongshe
刊名PLANT CELL TISSUE AND ORGAN CULTURE
出版日期2013
卷号113期号:2页码:245-256
关键词Abiotic stress DREB EMSA Promoter SAMDC
ISSN号0167-6857
DOI10.1007/s11240-012-0264-0
文献子类Article
英文摘要S-Adenosyl-methionine decarboxylase (SAMDC) and dehydration responsive element-binding proteins (DREBs) can improve plant resistance to abiotic stresses. These proteins have been extensively studied, but the mechanism for transcriptional regulation of SAMDC remains unclear. In this paper, the LcSAMDC2 gene and its promoter were isolated from Leymus chinensis. Two DRE cis-elements were identified from the promoter of LcSAMDC2 and shown to bind with LcDREB2. Subcellular localization and yeast one-hybrid assay revealed that LcDREB2 is a transcription factor. An electrophoretic mobility shift assay (EMSA) showed that LcDREB2 can bind to the LcSAMDC2 promoter probe containing a DRE element. Over-expression of LcDREB2 in L. chinensis callus increased expression of LcSAMDC2. Co-expression of LcDREB2 and the promoter of LcSAMDC2 fused with GUS in tobacco activated GUS activity. These results indicate that LcSAMDC2 is the downstream gene of LcDREB2. In addition, transgenic expression of LcDREB2 and LcSAMDC2 in Arabidopsis can improve the salt stress tolerance of transgenic lines. These results indicate that LcDREB2 cooperating with LcSAMDC2 contributes to resistance to abiotic stress.
学科主题Biotechnology & Applied Microbiology ; Plant Sciences
出版地DORDRECHT
电子版国际标准刊号1573-5044
WOS关键词S-ADENOSYLMETHIONINE DECARBOXYLASE ; POLYAMINES ; STRESS ; GENE ; GROWTH ; OVEREXPRESSION ; IDENTIFICATION ; BIOSYNTHESIS ; ETHYLENE ; LEAVES
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000317714300010
出版者SPRINGER
资助机构National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China (973)(National Basic Research Program of China)
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28078]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
2.Chinese Peoples Armed Police Force, Beijing Command Coll, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Peng, Xianjun,Zhang, Lexin,Zhang, Lixing,et al. The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2013,113(2):245-256.
APA Peng, Xianjun.,Zhang, Lexin.,Zhang, Lixing.,Liu, Zhujiang.,Cheng, Liqin.,...&Liu, Gongshe.(2013).The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis.PLANT CELL TISSUE AND ORGAN CULTURE,113(2),245-256.
MLA Peng, Xianjun,et al."The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis".PLANT CELL TISSUE AND ORGAN CULTURE 113.2(2013):245-256.

入库方式： OAI收割

来源：植物研究所

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