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Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation

文献类型：期刊论文

作者Liu, Hui1; Wang, Cuiping1; Komatsu, Setsuko2; He, Mingxia3; Liu, Gongshe; Shen, Shihua
刊名JOURNAL OF PROTEOMICS
出版日期2013
卷号91页码:23-40
关键词Jatropha curcas Proteomics Seed development Carbon flux Lipid accumulation
ISSN号1874-3919
DOI10.1016/j.jprot.2013.06.030
文献子类Article
英文摘要To characterize the metabolic signatures of lipid accumulation in Jatropha curcas seeds, comparative proteomic technique was employed to Profile protein changes during the seed development. Temporal changes in comparative proteome were examined using gels-based proteomic technique at six developmental stages for lipid accumulation. And 104 differentially expressed proteins were identified by MALDI-TOF/TOF tandem mass spectrometry. These protein species were classified into 10 functional categories, and the results demonstrated that protein species related to energy and metabolism were notably accumulated and involved in the carbon flux to lipid accumulation that occurs primarily from early to late stage in seed development. Glycolysis and oxidative pentose phosphate pathways were the major pathways of producing carbon flux, and the glucose-6-phosphate and triose-phosphate are the major carbon source for fatty acid synthesis. Lipid analysis revealed that fatty acid accumulation initiated 25 days after flowering at the late stage of seed development of J. curcas. Furthermore, C-16:0 was initially synthesized as the precursor for the elongation to C-18:1 and C-18:2 in the developing seeds of J. curcas. Together, the metabolic signatures on protein changes in seed development provide profound knowledge and perspective insights into understanding lipid network in J. curcas. Biological significance Due to the abundant oil content in seeds, Jatropha curcas seeds are being considered as the ideal materials for biodiesel. Although several studies had carried out the transcriptomic project to study the genes expression profiles in seed development of J. curcas, these ESTs hadn't been confirmed by qRT-PCR. Yet, the seed development of J. curcas had been described for a pool of developing seeds instead of being characterized systematically. Moreover, cellular metabolic events are also controlled by protein protein interactions, posttranslational protein modifications, and enzymatic activities which cannot be described by transcriptional profiling approaches alone. In this study, within the overall objective of profiling differential protein abundance in developing J. curcas seeds, we provide a setting of physiological data with dynamic proteomic and qRT-PCR analysis to characterize the metabolic pathways and the relationship between mRNA and protein patterns from early stage to seed filling during the seed development of J. curcas. The construction of J. curcas seed development proteome profiles will significantly increase our understanding of the process of seed development and provide a foundation to examine the dynamic changes of the metabolic network during seed development process and certainly suggest some clues to improve the lipid content off. curcas seeds. (C) 2013 Elsevier B.V. All rights reserved.
学科主题Biochemical Research Methods
出版地AMSTERDAM
电子版国际标准刊号1876-7737
WOS关键词CARRIER-PROTEIN DESATURASE ; FATTY-ACID SYNTHESIS ; ACETYL-COENZYME-A ; TRANSCRIPTOME ANALYSIS ; BRASSICA-NAPUS ; SYNTHASE-III ; OIL ; PLANT ; EXPRESSION ; BIODIESEL
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000327906000003
出版者ELSEVIER
资助机构National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences)
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/28086]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Res & Dev Resource Plant, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan
4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,et al. Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation[J]. JOURNAL OF PROTEOMICS,2013,91:23-40.
APA Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,He, Mingxia,Liu, Gongshe,&Shen, Shihua.(2013).Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation.JOURNAL OF PROTEOMICS,91,23-40.
MLA Liu, Hui,et al."Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation".JOURNAL OF PROTEOMICS 91(2013):23-40.

入库方式： OAI收割

来源：植物研究所

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