Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis)
文献类型：期刊论文
| 作者 | Su, Man1; Li, Xiaoxia1; Li, Xiaofeng; Cheng, Liqin; Qi, Dongmei; Chen, Shuangyan; Liu, Gongshe
|
| 刊名 | PLANT MOLECULAR BIOLOGY REPORTER
 |
| 出版日期 | 2013 |
| 卷号 | 31期号:5页码:1184-1191 |
| 关键词 | Sucrose transporter Molecular characterization Sheep grass Sucrose starvation Defoliation |
| ISSN号 | 0735-9640 |
| DOI | 10.1007/s11105-013-0582-3 |
| 文献子类 | Article |
| 英文摘要 | Sucrose is a major photosynthetic product in plants' leaves. Long-distance transport of sucrose requires sucrose transporter (SUT) to perform loading and unloading functions. In this study, a sucrose transporter gene LcSUT1 was cloned from sheep grass (Leymus chinensis (Trin.) Tzvel), a perennial grass of Gramineae: Poaceae. Bioinformatics analysis showed that the gene product LcSUT1 consisting of 12 predicted transmembrane domains and 11 loops belongs to the SUT1 clade. Heterologous expression of LcSUT1 in yeast proved that it was a functional sucrose transporter. Tissue-specific expression analysis showed that LcSUT1 was highly expressed in leaf and leaf sheath. The expression level of LcSUT1 was significantly up-regulated in leaf sheath after defoliation, but was not induced by wound signal. Furthermore, the level of LcSUT1 expression increased in callus, a model sink tissue, when grew on N6 medium without sucrose. Taken together, our results revealed a novel mechanism in which the increased expression of LcSUT1 in leaf sheaths after defoliation was caused by sucrose starvation rather than by wound signal. |
| 学科主题 | Biochemical Research Methods ; Plant Sciences |
| 出版地 | NEW YORK |
| 电子版国际标准刊号 | 1572-9818 |
| WOS关键词 | LOLIUM-PERENNE L ; SIEVE ELEMENTS ; LEAF SHEATHS ; REGROWTH ; CARBON ; FRUCTANS ; NITROGEN ; CARRIER ; FAMILY ; OSSUT1 |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000323669900014 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ningxia Agricultural Comprehensive Development Office ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences) ; National High Technology Research and Development Program of China (863)(National High Technology Research and Development Program of China) |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/28166]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Man,Li, Xiaoxia,Li, Xiaofeng,et al. Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis)[J]. PLANT MOLECULAR BIOLOGY REPORTER,2013,31(5):1184-1191. |
| APA | Su, Man.,Li, Xiaoxia.,Li, Xiaofeng.,Cheng, Liqin.,Qi, Dongmei.,...&Liu, Gongshe.(2013).Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis).PLANT MOLECULAR BIOLOGY REPORTER,31(5),1184-1191. |
| MLA | Su, Man,et al."Molecular Characterization and Defoliation-Induced Expression of a Sucrose Transporter LcSUT1 Gene in Sheep Grass (Leymus chinensis)".PLANT MOLECULAR BIOLOGY REPORTER 31.5(2013):1184-1191. |
入库方式： OAI收割
来源：植物研究所
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