Screening of alginate lyase-excreting microorganisms from the surface of brown algae
文献类型：期刊论文
| 作者 | Wang,Mingpeng5; Chen,Lei4; Zhang,Zhaojie3; Wang,Xuejiang2; Qin,Song4 ; Yan,Peisheng1,5
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| 刊名 | AMB Express
 |
| 出版日期 | 2017-04-04 |
| 卷号 | 7期号:1 |
| ISSN号 | 2191-0855 |
| 关键词 | Alginate lyase Gram’s iodine Alginate degradation Screening Brown algae |
| DOI | 10.1186/s13568-017-0361-x |
| 通讯作者 | Qin,Song(sqin@yic.ac.cn) ; Yan,Peisheng(yps6@163.com) |
| 英文摘要 | AbstractAlginate lyase is a biocatalyst that degrades alginate to produce oligosaccharides, which have many bioactive functions and could be used as renewable biofuels. Here we report a simple and sensitive plate assay for screening alginate lyase-excreting microorganisms from brown algae. Brown algae Laminaria japonica, Sargassum horneri and Sargassum siliquatrum were cultured in sterile water. Bacteria growing on the surface of seaweeds were identified and their capacity of excreting alginate lyase was analyzed. A total of 196 strains were recovered from the three different algae samples and 12 different bacterial strains were identified capable of excreting alginate lyases. Sequence analysis of the 16S rRNA gene revealed that these alginate lyase-excreting strains belong to eight genera: Paenibacillus (4/12), Bacillus (2/12), Leclercia (1/12), Isoptericola (1/12), Planomicrobium (1/12), Pseudomonas (1/12), Lysinibacillus (1/12) and Sphingomonas (1/12). Further analysis showed that the LJ-3 strain (Bacillus halosaccharovorans) had the highest enzyme activity. To our best knowledge, this is the first report regarding alginate lyase-excreting strains in Paenibacillus, Planomicrobium and Leclercia. We believe that our method used in this study is relatively easy and reliable for large-scale screening of alginate lyase-excreting microorganisms. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S13568-017-0361-X |
| 源URL | [http://ir.yic.ac.cn/handle/133337/32094]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Qin,Song; Yan,Peisheng |
| 作者单位 | 1.Harbin Institute of Technology at Weihai; School of Marine Science and Technology 2.Worldfull Agricultural Science and Technology Co., Ltd 3.University of Wyoming; Department of Zoology and Physiology 4.Yantai Institute of Costal Zone Research Chinese Academy of Sciences 5.Harbin Institute of Technology; School of Municipal and Environmental Engineering |
| 推荐引用方式 GB/T 7714 | Wang,Mingpeng,Chen,Lei,Zhang,Zhaojie,et al. Screening of alginate lyase-excreting microorganisms from the surface of brown algae[J]. AMB Express,2017,7(1). |
| APA | Wang,Mingpeng,Chen,Lei,Zhang,Zhaojie,Wang,Xuejiang,Qin,Song,&Yan,Peisheng.(2017).Screening of alginate lyase-excreting microorganisms from the surface of brown algae.AMB Express,7(1). |
| MLA | Wang,Mingpeng,et al."Screening of alginate lyase-excreting microorganisms from the surface of brown algae".AMB Express 7.1(2017). |
入库方式： OAI收割
来源：烟台海岸带研究所
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