A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula
文献类型：期刊论文
| 作者 | Yu, Zhenglin2; Qi, Aimin1; Wang, Lili1; Shan, Encui2; Li, Dongmei1; Yang, Xiaolong1; Zhang, Anguo1 |
| 刊名 | WATER
 |
| 出版日期 | 2022-11-01 |
| 卷号 | 14期号:21页码:12 |
| 关键词 | cytochrome P450 polychaetes Sternaspis sculata transcriptional response PAHs field study |
| DOI | 10.3390/w14213489 |
| 通讯作者 | Wang, Lili(llwang@nmemc.org.cn) |
| 英文摘要 | Polychaetes (such as Sternaspis sculata) can biotransform polycyclic aromatic hydrocarbons (PAHs) in the environment, and the cytochrome P450 (CYP) enzyme is the key substance in this process. Here, a novel CYP gene was identified from S. sculata, a marine polychaete that is abundantly distributed along marine coasts worldwide. The full-length cDNA of the new CYP is a 1829 bp, encoding a protein of 509 amino acids. The deduced amino acid sequence of the CYP gene contains the conserved motifs of the P450 family (FxxGxxxCxG) and the characteristic sequences of the CYP4 family (EVDTFMFEGHDTT), showing high similarity to CYP4V from marine polychaetes. Therefore, the cDNA sequence might belong to the subfamily of CYP4V, tentatively named SsCYP4V. The expression levels of SsCYP4V in the field were detected in S. sculata sampled from six sites along the coasts of the Liaodong Peninsula. The results showed that the relative expression levels of SsCYP4V in S. sculata were significantly different among the sampling sites; however, no positive relationship was found between SsCYP4V expression levels and PAHs concentrations in the sediments. These findings suggested a complexity of CYP gene expression in the field, and SsCYP4V cannot be used as a biomarker for the field monitoring of PAH pollution. |
| WOS关键词 | POLYCYCLIC AROMATIC-HYDROCARBONS ; CYTOCHROME-P450 GENES ; PERINEREIS-NUNTIA ; NEREIS-VIRENS ; EXPRESSION ; BIOACCUMULATION ; SEDIMENT ; P450 |
| WOS研究方向 | Environmental Sciences & Ecology ; Water Resources |
| 语种 | 英语 |
| WOS记录号 | WOS:000881525400001 |
| 资助机构 | National Natural Science Foundation of China ; National Marine Public Welfare Research Project ; Young Marine Science Foundation of SOA |
| 源URL | [http://ir.yic.ac.cn/handle/133337/32404]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Wang, Lili |
| 作者单位 | 1.Minist Ecol & Environm, Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Zhenglin,Qi, Aimin,Wang, Lili,et al. A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula[J]. WATER,2022,14(21):12. |
| APA | Yu, Zhenglin.,Qi, Aimin.,Wang, Lili.,Shan, Encui.,Li, Dongmei.,...&Zhang, Anguo.(2022).A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula.WATER,14(21),12. |
| MLA | Yu, Zhenglin,et al."A Novel CYP4 Gene Identified in the Polychaete Sternaspis scutata and Its Transcriptional Levels along the Coasts of the Liaodong Peninsula".WATER 14.21(2022):12. |
入库方式： OAI收割
来源：烟台海岸带研究所
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