Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting
文献类型：期刊论文
| 作者 | A. L. Li; K. Z. Chen; B. Li; P. Liang and C. F. Shen |
| 刊名 | Bulletin of Environmental Contamination and Toxicology
 |
| 出版日期 | 2022 |
| 卷号 | 109期号:3页码:571-576 |
| ISSN号 | 0007-4861 |
| DOI | 10.1007/s00128-022-03574-8 |
| 英文摘要 | In this work, biphenyl was used as carbon source to enrich microorganisms from polychlorinated biphenyls (PCBs)-contaminated paddy soil samples, and the taxonomic structures in both of the soil samples and the fourth-generation enrichments were examined with high-throughput sequencing. Single cells were isolated from the enrichments via single cell sorting technology named Laser Induced Visualized Ejection Separation Technology (LIVEST) and also traditional single colony sorting, and the genera of the isolates were identified using 16S rRNA sequencing. The results from high-throughput sequencing present that enrichment from generation to generation can considerably change the microbial community. Comparing the two sorting methods, the LIVEST is more time-saving and cell-targeted for microbial resource exploration. Based on the further verification of biphenyl degradation, it was found that some strains belonging to genera Macrococcus, Aerococcus and Metabacillus are capable in degrading biphenyl, which have not been reported yet. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/66441]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | A. L. Li,K. Z. Chen,B. Li,et al. Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting[J]. Bulletin of Environmental Contamination and Toxicology,2022,109(3):571-576. |
| APA | A. L. Li,K. Z. Chen,B. Li,&P. Liang and C. F. Shen.(2022).Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting.Bulletin of Environmental Contamination and Toxicology,109(3),571-576. |
| MLA | A. L. Li,et al."Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting".Bulletin of Environmental Contamination and Toxicology 109.3(2022):571-576. |
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