Bioinspired Design of Reversible Fluorescent Probes for Tracking Nitric Oxide Dynamics in Live Cells
文献类型：期刊论文
| 作者 | Guo Ruiying3; Zhang Yutian2; Chanmungkalakul Supphachok1; Guo Haoran2; Hu Yongzhou3; Li Jia2 ; Liu Xiaogang1; Zang Yi2; Li Xin3
|
| 刊名 | CCS CHEMISTRY
 |
| 出版日期 | 2021 |
| 卷号 | 3期号:10页码:180 |
| 关键词 | fluorescent probe fluorescence imaging reversible nitric oxide S-nitrosation |
| ISSN号 | 2096-5745 |
| 英文摘要 | Nitric oxide (NO) participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology. While there are methods available for detecting biological NO, few are capable of tracking NO dynamics. Herein, inspired by the cellular machinery of reversible thiol modification by NO, we have successfully designed a family of cysteine analogues tagged with fluorophores for visualizing cellular NO dynamics. Biocompatible probes la and 2a were found to be most tolerated by the cellular machinery to swiftly switch between their original fluorescent state and quenched S-nitrosated state in response to cellular NO dynamics. We showcased their applicability in various cell types and utility to track NO fluctuations in activated murine macrophages in response to an anti-inflammatory agent treatment. We expect that probes la and 2a will afford promising tools for studying NO pathophysiology by tracking its dynamics in health and disease. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/304797]  |
| 专题 | 中国科学院上海药物研究所 |
| 作者单位 | 1.新加坡科技与设计大学 2.中国科学院上海药物研究所 3.浙江大学 |
| 推荐引用方式 GB/T 7714 | Guo Ruiying,Zhang Yutian,Chanmungkalakul Supphachok,et al. Bioinspired Design of Reversible Fluorescent Probes for Tracking Nitric Oxide Dynamics in Live Cells[J]. CCS CHEMISTRY,2021,3(10):180. |
| APA | Guo Ruiying.,Zhang Yutian.,Chanmungkalakul Supphachok.,Guo Haoran.,Hu Yongzhou.,...&Li Xin.(2021).Bioinspired Design of Reversible Fluorescent Probes for Tracking Nitric Oxide Dynamics in Live Cells.CCS CHEMISTRY,3(10),180. |
| MLA | Guo Ruiying,et al."Bioinspired Design of Reversible Fluorescent Probes for Tracking Nitric Oxide Dynamics in Live Cells".CCS CHEMISTRY 3.10(2021):180. |
入库方式： OAI收割
来源：上海药物研究所
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