3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis
文献类型:期刊论文
作者 | Kang,Yue2,3; Xu,Jie3,4; Meng,Ling’ao2; Su,Ya3; Fang,Huan3,4; Liu,Jiaqi3; Cheng,Yuen Yee1; Jiang,Daqing2; Nie,Yi4,5; Song,Kedong3 |
刊名 | Biofabrication |
出版日期 | 2023-02-09 |
卷号 | 15期号:2 |
ISSN号 | 1758-5082 |
关键词 | Exosome Angiogenesis Osteogenic Quaterinized Chitosan Antibacterial |
DOI | 10.1088/1758-5090/acb6b8 |
英文摘要 | Abstract Craniofacial bone regeneration is a coupled process of angiogenesis and osteogenesis, which, associated with infection, still remains a challenge in bone defects after trauma or tumor resection. 3D tissue engineering scaffolds with multifunctional-therapeutic properties can offer many advantages for the angiogenesis and osteogenesis of infected bone defects. Hence, in the present study, a microchannel networks-enriched 3D hybrid scaffold composed of decellularized extracellular matrix (dECM), gelatin (Gel), quaterinized chitosan (QCS) and nano-hydroxyapatite (nHAp) (dGQH) was fabricated by an extrusion 3D bioprinting technology. And enlightened by the characteristics of natural bone microstructure and the demands of vascularized bone regeneration, the exosomes (Exos) isolated from human adipose derived stem cells as angiogenic and osteogenic factors were then co-loaded into the desired dGQH20 hybrid scaffold based on an electrostatic interaction. The results of the hybrid scaffolds performance characterization showed that these hybrid scaffolds exhibited an interconnected pore structure and appropriate degradability (>61% after 8 weeks of treatment), and the dGQH20 hybrid scaffold displayed the highest porosity (83.93 ± 7.38%) and mechanical properties (tensile modulus: 62.68 ± 10.29 MPa, compressive modulus: 16.22 ± 3.61 MPa) among the dGQH hybrid scaffolds. Moreover, the dGQH20 hybrid scaffold presented good antibacterial activities (against 94.90 ± 2.44% of Escherichia coli and 95.41 ± 2.65% of Staphylococcus aureus, respectively) as well as excellent hemocompatibility and biocompatibility. Furthermore, the results of applying the Exos to the dGQH20 hybrid scaffold showed that the Exo promoted the cell attachment and proliferation on the scaffold, and also showed a significant increase in osteogenesis and vascularity regeneration in the dGQH@Exo scaffolds in vitro and in vivo. Overall, this novel dECM/Gel/QCS/nHAp hybrid scaffold laden with Exo has a considerable potential application in reservation of craniofacial bone defects. |
语种 | 英语 |
出版者 | IOP Publishing |
WOS记录号 | IOP:BF_15_2_024103 |
源URL | [http://ir.ipe.ac.cn/handle/122111/56618] |
通讯作者 | Jiang,Daqing; Nie,Yi; Song,Kedong |
作者单位 | 1.Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia 2.Department of Breast Surgery, Cancer Hospital of Dalian University of Technology, Liaoning Cancer Hospital and Institute, Shenyang 110042, People’s Republic of China 3.State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, People’s Republic of China 4.Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou 450000, People’s Republic of China 5.Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China |
推荐引用方式 GB/T 7714 | Kang,Yue,Xu,Jie,Meng,Ling’ao,et al. 3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis[J]. Biofabrication,2023,15(2). |
APA | Kang,Yue.,Xu,Jie.,Meng,Ling’ao.,Su,Ya.,Fang,Huan.,...&Song,Kedong.(2023).3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis.Biofabrication,15(2). |
MLA | Kang,Yue,et al."3D bioprinting of dECM/Gel/QCS/nHAp hybrid scaffolds laden with mesenchymal stem cell-derived exosomes to improve angiogenesis and osteogenesis".Biofabrication 15.2(2023). |
入库方式: OAI收割
来源:过程工程研究所
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