Evaluating the Degradation Process of Collagen Sponge and Acellular Matrix Implants In Vivo Using the Standardized HPLC-MS/MS Method
文献类型：期刊论文
| 作者 | Gao, Jianping1,2; Ma, Ye1; Guo, Zhenhu1; Zhang, Yang1; Xing, Fangyu1; Zhang, Tianyang1; Kong, Yingjun1,2; Luo, Xi1,2; Xu, Liming3; Zhang, Guifeng1,2 |
| 刊名 | SEPARATIONS
 |
| 出版日期 | 2023 |
| 卷号 | 10期号:1页码:16 |
| 关键词 | in vivo degradation process collagen sponge acellular matrix HPLC-MS MS |
| DOI | 10.3390/separations10010047 |
| 英文摘要 | The purpose of this study was to establish a collagen determination method based on an isotope-labeled collagen peptide as an internal reference via high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and using the established method to evaluate the degradation process of collagen-based implants in vivo. The specific peptide (GPAGPQGPR) of bovine type I collagen was identified with an Orbitrap mass spectrometer. Then, the quantification method based on the peptide detection with HPLC-MS/MS was established and validated, and then further used to analyze the degradation trend of the collagen sponge and acellular matrix (ACM) in vivo at 2, 4, 6, 8, 12, 16, and 18 weeks after implantation. The results indicate that the relative standard deviation (RSD) of the detection precision and repeatability of the peptide-based HPLC-MS/MS quantification method were 3.55% and 0.63%, respectively. The limitations of quantification and detection were 2.05 x 10(-3) mu g/mL and 1.12 x 10(-3) mu g/mL, respectively. The collagen sponge and ACM were completely degraded at 10 weeks and 18 weeks, respectively. Conclusion: A specific peptide (GPAGPQGPR) of bovine type I collagen was identified with an Orbitrap mass spectrometer, and a standardized HPLC-MS/MS-based internal reference method for the quantification of bovine type I collagen was established. The method can be used for the analysis of the degradation of collagen-based implants in vivo. |
| WOS关键词 | DERMAL MATRIX ; III COLLAGEN ; QUANTIFICATION ; HYDROXYPROLINE ; SCAFFOLDS ; RECOVERY ; DEFECTS ; CELLS ; SKIN |
| 资助项目 | National Key Technology R&D Programs of China[2021YFC2400801] ; Science and Technology Program of Guangzhou, China[201803010086] ; Open Funding Project of the State Key Laboratory of Biochemical Engineering[2021KF-04] ; Independent Research Project of the State Key Laboratory of Biochemical Engineering[2021ZZ-03] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000918899500001 |
| 资助机构 | National Key Technology R&D Programs of China ; Science and Technology Program of Guangzhou, China ; Open Funding Project of the State Key Laboratory of Biochemical Engineering ; Independent Research Project of the State Key Laboratory of Biochemical Engineering |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/56696]  |
| 通讯作者 | Zhang, Guifeng |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem & Engn, Beijing 100049, Peoples R China 3.Natl Inst Food & Drug Control, Beijing 100050, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Jianping,Ma, Ye,Guo, Zhenhu,et al. Evaluating the Degradation Process of Collagen Sponge and Acellular Matrix Implants In Vivo Using the Standardized HPLC-MS/MS Method[J]. SEPARATIONS,2023,10(1):16. |
| APA | Gao, Jianping.,Ma, Ye.,Guo, Zhenhu.,Zhang, Yang.,Xing, Fangyu.,...&Zhang, Guifeng.(2023).Evaluating the Degradation Process of Collagen Sponge and Acellular Matrix Implants In Vivo Using the Standardized HPLC-MS/MS Method.SEPARATIONS,10(1),16. |
| MLA | Gao, Jianping,et al."Evaluating the Degradation Process of Collagen Sponge and Acellular Matrix Implants In Vivo Using the Standardized HPLC-MS/MS Method".SEPARATIONS 10.1(2023):16. |
入库方式： OAI收割
来源：过程工程研究所
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