NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a "One-Stone-Two-Birds" manner
文献类型：期刊论文
| 作者 | Wu, Guanghao2; Su, Qihang3; Li, Jie1; Xue, Chao3; Zhu, Jie4; Cai, Qiuchen3; Huang, Jingbiao3; Ji, Shaoyang4; Cheng, Biao5; Ge, Hengan5 |
| 刊名 | JOURNAL OF NANOBIOTECHNOLOGY
 |
| 出版日期 | 2023-01-05 |
| 卷号 | 21期号:1页码:20 |
| 关键词 | Extracellular vesicles Adipose tissue-derived mesenchymal stem cells NAD plus metabolism Macrophage Tendinopathy |
| DOI | 10.1186/s12951-022-01763-5 |
| 英文摘要 | Background Tendinopathy is the leading sports-related injury and will cause severe weakness and tenderness. Effective therapy for tendinopathy remains limited, and extracellular vesicles (EVs) derived from adipose tissue-derived mesenchymal stem cells (ADMSCs) have demonstrated great potential in tendinopathy treatment; however, the influence of aging status on EV treatment has not been previously described. Results In this study, it was found that ADMSCs derived from old mice (ADMSC(old)) demonstrated remarkable cellular senescence and impaired NAD+ metabolism compared with ADMSCs derived from young mice (ADMSC(young)). Lower NAMPT contents were detected in both ADMSCold and its secreted EVs (ADMSC(old)-EVs). Advanced animal experiments demonstrated that ADMSC(young)-EVs, but not ADMSC(old)-EVs, alleviated the pathological structural, functional and biomechanical properties in tendinopathy mice. Mechanistic analyses demonstrated that ADMSC(young)-EVs improved cell viability and relieved cellular senescence of tenocytes through the NAMPT/SIRT1/PPAR gamma/PGC-1 alpha path-way. ADMSC(young)-EVs, but not ADMSC(old)-EVs, promoted phagocytosis and M2 polarization in macrophages through the NAMPT/SIRT1/Nf-kappa b p65/NLRP3 pathway. The macrophage/tenocyte crosstalk in tendinopathy was influenced by ADMSC(young)-EV treatment and thus it demonstrated "One-Stone-Two-Birds " effects in tendinopathy treatment.Conclusions This study demonstrates an effective novel therapy for tendinopathy and uncovers the influence of donor age on curative effects by clarifying the detailed biological mechanism. |
| WOS关键词 | BM-MSCS ; AD-MSCS ; RATS |
| 资助项目 | National Natural Science Foundation of China[81972444] ; National Natural Science Foundation of China[81972095] ; Project of Shanghai Science and Technology Commission[20S31901400] ; Project of Shanghai Science and Technology Commission[19441901700] ; Project of Shanghai Science and Technology Commission[19441901701] ; Project of Shanghai Science and Technology Commission[19441901702] ; Project of Shanghai Health Commission[20194Y0385] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000912376600001 |
| 出版者 | BMC |
| 资助机构 | National Natural Science Foundation of China ; Project of Shanghai Science and Technology Commission ; Project of Shanghai Health Commission |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/56801]  |
| 通讯作者 | Ji, Shaoyang; Cheng, Biao; Ge, Hengan |
| 作者单位 | 1.Zhabei Cent Hosp Jingan Dist, Dept Orthoped, Shanghai 200070, Peoples R China 2.Beijing Inst Technol, Sch Mat Sci & Engn, Beijing 100081, Peoples R China 3.Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Orthoped, Shanghai 200072, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 5.Tongji Univ, Tongji Hosp, Sch Med, Dept Orthoped, Shanghai 200065, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Guanghao,Su, Qihang,Li, Jie,et al. NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a "One-Stone-Two-Birds" manner[J]. JOURNAL OF NANOBIOTECHNOLOGY,2023,21(1):20. |
| APA | Wu, Guanghao.,Su, Qihang.,Li, Jie.,Xue, Chao.,Zhu, Jie.,...&Ge, Hengan.(2023).NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a "One-Stone-Two-Birds" manner.JOURNAL OF NANOBIOTECHNOLOGY,21(1),20. |
| MLA | Wu, Guanghao,et al."NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a "One-Stone-Two-Birds" manner".JOURNAL OF NANOBIOTECHNOLOGY 21.1(2023):20. |
入库方式： OAI收割
来源：过程工程研究所
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