Purification of plasmid DNA from escherichia coli lysate using superparamagnetic nanoparticles modified with amino functional groups
文献类型：会议论文
| 作者 | Qiu, X.L.1; Guan, Y.P.1; Wang, K.K.1; Guo, C.2 |
| 出版日期 | 1905-07-08 |
| 会议日期 | May 29, 2015 - May 31, 2015 |
| 会议地点 | Incheon, Korea, Republic of |
| 关键词 | Ionic strength - Escherichia coli - DNA - Nanoparticles - Magnetite - Superparamagnetism - Biomolecules |
| 页码 | 387-391 |
| 英文摘要 | Aiming at enhancing the efficiency and yield of purification, plasmid DNA purification by amine-functionalized Fe3O4 superparamagnetic nanoparticles was studied in this paper. Due to the large specific surface area, the adsorption capacity of these nanoparticles was found to be 447.3 mg/g, which have never been achieved with Fe3O4 particles whose average size is larger than 30 nm. The effects of ionic strength, pH and eluent mode during the elution process were investigated, and the yield was increased to 24.7 mg/g when an ultrasonic concussion was used. 漏 2016 Taylor & Francis Group, London. |
| 项目编号 | This study was financially supported by the National Natural Science Foundation of China (51274035) and the National Basic Research Program of China (2013CB632602). |
| 资助机构 | CRC Press/Balkema |
| 会议录 | International Conference on Advanced Materials, Structures and Mechanical Engineering, ICAMSME 2015
 |
| 学科主题 | Purification |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/59327]  |
| 作者单位 | 1.School of Materials Science and Engineering, University of Science and Technology Beijing, USTB, Beijing, China 2.Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China |
| 推荐引用方式 GB/T 7714 | Qiu, X.L.,Guan, Y.P.,Wang, K.K.,et al. Purification of plasmid DNA from escherichia coli lysate using superparamagnetic nanoparticles modified with amino functional groups[C]. 见:. Incheon, Korea, Republic of. May 29, 2015 - May 31, 2015. |
入库方式： OAI收割
来源：过程工程研究所
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