Electrospun poly (styrene-co-maleic anhydride) nanofibers for 尾-D-galactosidase immobilization and enzymatic transgalactosylation
文献类型：会议论文
| 作者 | Li, Pengju2,3; Zhang, Yufei1; Zhang, Songping1; Wang, Ping1; Wang, Min2 |
| 出版日期 | 1905-07-04 |
| 会议日期 | December 23, 2011 - December 25, 2011 |
| 会议地点 | Beihai, China |
| 关键词 | Nonionic surfactants - Enzyme activity - Chlorine compounds - Lithium compounds - Styrene - Maleic anhydride |
| 卷号 | 396-398 |
| DOI | 10.4028/www.scientific.net/AMR.396-398.1394 |
| 页码 | 1394-1397 |
| 英文摘要 | Poly(styrene-co-maleic anhydride) nanofibers with a uniform diameter of about 400 nm were prepared by eletrospinning a solution containing 50 % (w/v) polymer and 1% (w/v) LiCl, 25 cm gap between jection needle tip and collector, a flow rate of 200 渭L/h, and an applied voltage of 24 kV. 尾-D- galactosidase was immobilized to the electrospun nanofibers and subsequently used for synthesizing hexyl-galactoside, an important nonionic surfactants, by catalyzing the transgalactosylation reaction between hexanol and lactose. Co-electrospun LiCl was found leading to a significant enhancement in the enzyme loading and activity of the immobilized 尾-D-galactosidase compared to nanofiber without LiCl. After 7 reusing cycles at 37掳C, the immobilized enzyme retained about 33% of its original activity. |
| 资助机构 | Trans Tech Publications, P.O. Box 1254, Clausthal-Zellerfeld, D-38670, Germany |
| 会议录 | 2011 International Conference on Chemical, Material and Metallurgical Engineering, ICCMME 2011
 |
| 学科主题 | Nanofibers |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/59472]  |
| 作者单位 | 1.National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China 2.Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin 300457, China 3.College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China |
| 推荐引用方式 GB/T 7714 | Li, Pengju,Zhang, Yufei,Zhang, Songping,等. Electrospun poly (styrene-co-maleic anhydride) nanofibers for 尾-D-galactosidase immobilization and enzymatic transgalactosylation[C]. 见:. Beihai, China. December 23, 2011 - December 25, 2011. |
入库方式： OAI收割
来源：过程工程研究所
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