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Hydrophilically coated gigaporous polystyrene microspheres and their application in high-speed protein chromatography

文献类型：会议论文

作者Qu, Jian Bo1; Zhang, Xiao Jianbo Xiao1; Jing, Guanglun1; Zhou, Weiqing2; Ma, Guanghui2
出版日期1905-07-03
会议日期May 28, 2011 - May 30, 2011
会议地点Changsha, China
关键词Flow velocity - Ion exchangers - Chromatography - Hydrophilicity - Styrene - Polystyrenes - Proteins - Coatings
卷号233-235
DOI10.4028/www.scientific.net/AMR.233-235.1924
页码1924-1929
英文摘要Gigaporous poly (styrene-divinylbenzene) (PS) microspheres have been effectively hydrophilized by coating with hydrophobically modified agarose (phenoxyl agarose, Agap). The effect of the phenoxy content on the amount of Agap adsorbed onto the PS surface was examined and various samples of microspheres, differing in surface Agap density, were prepared. After coating, the hydrophilicity of gigaporous PS microspheres was found to be greatly enhanced and the gigaporous character of PS microspheres was well maintained. The modified particles were further coupled with DEAE groups to prepare a DEAE anion exchange medium (DEAE-AP). A column packed with DEAE-AP showed low back pressure and high protein resolution at high flow velocity. Notably, the DEAE-AP column could completely separate a model protein mixture at a flow velocity of 3612 cm/h within 2 min. The results indicate that the gigaporous matrix has considerable advantages for high-speed protein chromatography. 漏 (2011) Trans Tech Publications, Switzerland.
资助机构Trans Tech Publications, P.O. Box 1254, Clausthal-Zellerfeld, D-38670, Germany
会议录2011 International Conference on Chemical Engineering and Advanced Materials, CEAM 2011
学科主题Microspheres
源URL[http://ir.ipe.ac.cn/handle/122111/59480]  
作者单位1.Center for Bioengineering and Biotechnology, China University of Petroleum (East China), QingDao 266555, China
2.National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
推荐引用方式
GB/T 7714		 Qu, Jian Bo,Zhang, Xiao Jianbo Xiao,Jing, Guanglun,et al. Hydrophilically coated gigaporous polystyrene microspheres and their application in high-speed protein chromatography[C]. 见:. Changsha, China. May 28, 2011 - May 30, 2011.

入库方式： OAI收割

来源：过程工程研究所

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