Glycerol-assisted hydrophobic interaction chromatography improving refolding of recombinant human granulocyte colony-stimulating factor
文献类型：会议论文
| 作者 | Wang, Fangwei1; Liu, Yongdong2; Ma, Guanghui2; Su, Zhiguo2 |
| 出版日期 | 2009-12-01 |
| 关键词 | Hybrid integrated circuits - Liquid chromatography - Glycerol - Hydrophobicity - Hydrophobic chromatography - Recombinant proteins |
| 卷号 | 159 |
| 期号 | 3 |
| DOI | 10.1007/s12010-008-8495-6 |
| 页码 | 634-641 |
| 英文摘要 | Efficient refolding of recombinant proteins in the forms of inclusion bodies at higher concentration remains challenging. Here, we report a strategy of a dual-gradient hydrophobic interaction chromatography (HIC) mode to refold recombinant human granulocyte colony-stimulating factor from its inclusion bodies at high protein concentration. The strategy was taken to meet the demand of dynamic refolding proceeding by gradually decrease the denaturant (guanidine-HCl) concentration and gradually increase the hydrophilicity of media (column of Poros PE 20) with glycerol as additive to provide a mild refolding surroundings. Compared with dilution method, this dual-gradient HIC process gave about 8.5-fold of increase in specific activity and 30% increase in soluble protein recovery. Furthermore, much higher protein concentration could be obtained at the same time. |
| 项目编号 | Acknowledgments The authors are thankful for the financial support from the Natural Science Foundation of China (Contract Nos. 20636010, 20576136, and 20820102036), the National 863 High-Tech Project (Contract No. 2007AA021604), and the National 973 High-Tech Project (Contract No. 2007CB714305) |
| 资助机构 | Humana Press Inc. |
| 学科主题 | Hydrophilicity |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/59521]  |
| 作者单位 | 1.Second Clinical College, Dalian Medical University, Dalian 116027, China 2.State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China |
| 推荐引用方式 GB/T 7714 | Wang, Fangwei,Liu, Yongdong,Ma, Guanghui,et al. Glycerol-assisted hydrophobic interaction chromatography improving refolding of recombinant human granulocyte colony-stimulating factor[C]. 见:. |
入库方式： OAI收割
来源：过程工程研究所
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