Preparation of uniformly sized agarose microcapsules by membrane emulsification for application in sorting bacteria
文献类型：会议论文
| 作者 | Zhou, Qing-Zhu1; Liu, Xing-Yu2; Liu, Shuang-Jiang2; Guang-Hui, Ma1; Su, Zhi-Guo1 |
| 出版日期 | 2008-09-03 |
| 关键词 | Pore size - Cells - Emulsification - Cultivation - Encapsulation - Solutions - Throughput - Cell culture - Membranes - Microstructure |
| 卷号 | 47 |
| 期号 | 17 |
| DOI | 10.1021/ie800011r |
| 页码 | 6386-6390 |
| 英文摘要 | In an attempt to develop a high-throughput culture process, bacterial cells were encapsulated in uniformsized agarose microcapsules prepared by membrane emulsification method. A mixture of diluted bacterial cell suspensions and agarose aqueous solution (40C) was used as the water phase, and a mixture of liquid paraffin and petroleum ether that contained hexaglycerin penta ester (PO-500) was used as the oil phase. The water phase permeated through uniform membrane pores into the oil phase under the pressure of nitrogen gas to form a uniform water/oil (W/O) emulsion; the emulsion then was cooled to form uniformly sized gel capsules. The size of microcapsules can be controlled by membranes with different pore sizes, because the mean diameters of the capsules were proportional to the mean pore diameters of the membranes. These microcapsules, which contained bacterial cells, were transferred into a column vessel and were incubated for 14 days to form microcolonies within the microcapsules. The cultured microcapsules were analyzed by flow cytometry, and the microbial colonized capsules were separated from free-living cells and empty capsules, based on the distinctive light-scattering signature of the microcapsules. The result showed that membrane emulsification preserved the viability of cells and this method was effective to create niches for various bacterial cells growing in high-throughput cultivation vessels. 漏 2008 American Chemical Society. |
| 资助机构 | American Chemical Society |
| 学科主题 | Light Scattering |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/59545]  |
| 作者单位 | 1.Stoe Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China 2.Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
| 推荐引用方式 GB/T 7714 | Zhou, Qing-Zhu,Liu, Xing-Yu,Liu, Shuang-Jiang,et al. Preparation of uniformly sized agarose microcapsules by membrane emulsification for application in sorting bacteria[C]. 见:. |
入库方式： OAI收割
来源：过程工程研究所
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