Lipase immobilization on oleic acid-pluronic (L-64) block copolymer coated magnetic nanoparticles, for hydrolysis at the oil/water interface
文献类型：会议论文
| 作者 | Mahmood, Iram1,2; Guo, Chen1; Xia, Hansong1,2; Junhe, Ma.1; Jiangs, Yangyang1,2; Liu, Huizhou1 |
| 出版日期 | 2008-09-03 |
| 关键词 | Fourier transform infrared spectroscopy - Oleic acid - High resolution transmission electron microscopy - Nanomagnetics - Enzyme immobilization - Nuclear magnetic resonance - X ray diffraction - Block copolymers - Hydrophobicity - Interfaces (materials) - Metal nanoparticles - Saturation magnetization |
| 卷号 | 47 |
| 期号 | 17 |
| DOI | 10.1021/ie701788x |
| 页码 | 6379-6385 |
| 英文摘要 | Here, we have reported a new approach for utilizing oleic acid-Pluronic L-64 block copolymer coated iron oxide nanoparticles as supports for enzyme immobilization. Iron oxide nanoparticles were prepared by a coprecipitation method and were coated with oleic acid and Pluronic to achieve higher stability and dispersibility. The surface morphology and size of the particle, as determined by transmission electron microscopy (TEM), was 卤10 nm. X-ray diffraction (XRD) patterns were taken over a range from 10 to 90 20, using Cu K radiation. Saturation magnetization values, measured at 300 K, varied from 34.6 emu/g to 60.8 emu/g. The possible interaction behavior of oleic acid and Pluronic was observed by Fourier transform infrared (FTIR) analysis and nuclear magnetic resonance (NMR) studies. Further potential of this material as a support for lipase immobilization and enzymatic hydrolysis at the oil/water interface was also investigated. The features of the surface-coated magnetic particles enable the adsorption of lipase from Candida cylindraces via strong hydrophobic interactions, which enhances the stability of the adsorbed enzyme molecules. The stability of the catalyst and, hence, its industrial applicability was tested by performing subsequent reaction cycles for the hydrolysis of olive oil. The activity of the immobilized lipase, pretreated with its substrate, was 510 U/g-matrix and was observed to be maintained at levels as high as 90% of its original activity for up to at least seven reuses. 漏 2008 American Chemical Society. |
| 资助机构 | American Chemical Society |
| 学科主题 | Iron Oxides |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/59557]  |
| 作者单位 | 1.Laboratory of Separation Sciences and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Zhongguancun N I, St 2, Beijing 100080, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Mahmood, Iram,Guo, Chen,Xia, Hansong,et al. Lipase immobilization on oleic acid-pluronic (L-64) block copolymer coated magnetic nanoparticles, for hydrolysis at the oil/water interface[C]. 见:. |
入库方式： OAI收割
来源：过程工程研究所
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