Conjugation of haloalkane dehalogenase DhaA with arabinogalactan to increase its stability
文献类型：期刊论文
| 作者 | Wang, Meiqi1,3; Yu, Weili3; Shen, Lijuan3; Zheng, He2; Guo, Xuan2; Zhong, Jinyi2; Hu, Tao3 |
| 刊名 | Journal of Biotechnology
 |
| 出版日期 | 2021-07-20 |
| 卷号 | 335页码:47-54 |
| 关键词 | Sodium chloride |
| ISSN号 | 1681656 |
| DOI | 10.1016/j.jbiotec.2021.06.002 |
| 英文摘要 | Haloalkane dehalogenase DhaA can catalyze the hydrolytic cleavage of carbonhalogen bonds, along with production of the corresponding alcohol, a proton and a halide. However, DhaA suffers from poor environmental tolerance, such as sensitivity to high temperature, low pH and hypersaline. Arabinogalactan (AG) is a hydrophilic polysaccharide with highly branched long chains. DhaA was conjugated with AG to improve the environmental stability of DhaA in the present study. Each DhaA was averagely conjugated with 4鈭? AG molecules. Conjugation of AG essentially maintained the enzymatic activity of DhaA (91.4 %) without apparent structural alteration. The hydration layer formed by AG could reduce the solvent accessible area of DhaA and slow the protonation process, thereby improving the pH and high salt stability of DhaA. In particular, the remaining activities of the conjugate (AG-DhaA) were 35.3 % after treatment at pH4.0 for 1 h, and 80.8 % in 1 M NaCl after treatment for 16 h. As compared with DhaA, AG-DhaA showed slightly different kinetic parameters (K M of 1.90 渭mol/L and k cat of 2.60 s -1). 漏 2021 Elsevier B.V. |
| 学科主题 | Stability |
| 项目编号 | This study was financially supported by National Natural Science Foundation of China ( 31970875 ), and National Key Research and Development Project of China ( 2018YFA0900804 ). |
| 出版者 | Elsevier B.V. |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/60282]  |
| 作者单位 | 1.University of Chinese Academy of Sciences, Beijing; 100190, China 2.State Key Laboratory of NBC Protection for Civilian, Research Institute of Chemical Defense, Beijing; 102205, China 3.State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing; 100190, China |
| 推荐引用方式 GB/T 7714 | Wang, Meiqi,Yu, Weili,Shen, Lijuan,et al. Conjugation of haloalkane dehalogenase DhaA with arabinogalactan to increase its stability[J]. Journal of Biotechnology,2021,335:47-54. |
| APA | Wang, Meiqi.,Yu, Weili.,Shen, Lijuan.,Zheng, He.,Guo, Xuan.,...&Hu, Tao.(2021).Conjugation of haloalkane dehalogenase DhaA with arabinogalactan to increase its stability.Journal of Biotechnology,335,47-54. |
| MLA | Wang, Meiqi,et al."Conjugation of haloalkane dehalogenase DhaA with arabinogalactan to increase its stability".Journal of Biotechnology 335(2021):47-54. |
入库方式： OAI收割
来源：过程工程研究所
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