Novel enzyme-metal-organic framework composite for efficient cadaverine production
文献类型：期刊论文
| 作者 | Yao, Jiahao1,2; Li, Zhuang2; Ji, Xiuling2; Xue, Yaju2; Ren, Baozeng1; Zhao, Hai5; Huang, Yuhong2,3,4,5 |
| 刊名 | Biochemical Engineering Journal
 |
| 出版日期 | 2021-12-01 |
| 卷号 | 176 |
| ISSN号 | 1369703X |
| 关键词 | Metal-Organic Frameworks - Amino acids - Enzyme immobilization |
| DOI | 10.1016/j.bej.2021.108222 |
| 英文摘要 | Cadaverine is an important precursor for the synthesis of nylon 5X, which is used for engineering plastics and fibers. L-lysine can be converted to cadaverine by lysine decarboxylases with high catalytic activity and selectivity. However, the pH sensitivity and non-recyclability impede the practical application of lysine decarboxylases for cadaverine production. In this study, lysine decarboxylase (LdcEt) was immobilized on the surface of the zirconium-based metal-organic framework material (MOF) UIO-66-NH2 via covalent linkage for the first time. Morphological and structural analysis of the enzyme-MOF composite (LdcEt@UIO-66-NH2) indicated that LdcEt was successfully immobilized on the surface of UIO-66-NH2. Meanwhile, LdcEt@UIO-66-NH2 showed higher catalytic efficiency and enzyme activity, broader pH and temperature reaction range than free LdcEt. Compared with free enzymes, LdcEt@UIO-66-NH2 could catalyze more cadaverine in 2 h at high-level substrate concentration (365.3 g L鈭?). Furthermore, LdcEt@UIO-66-NH2 can also be reused for 10 cycles. These results proved that the efficient enzyme-MOF composite LdcEt@UIO-66-NH2 has great potential for the large scale cadaverine production. 漏 2021 Elsevier B.V. |
| 学科主题 | Catalyst Activity |
| 项目编号 | This work was supported by the National Natural Science Foundation of China (grant number 22078346 ) ; the Beijing Nova Program of Science and Technology (grant number Z201100006820141 ) ; the Innovation Academy for Green Manufacture, CAS (grant number IAGM2020C19 ) ; the Henan Key Research and Development Project (grant number 202102210046 ) ; the Hebei Natural Science Foundation (grant number B2020103010 ) ; and the CAS Pioneer Hundred Program . Sincerely appreciate Prof. Suojiang Zhang (IPE, CAS) for his careful academic guidance and great support. |
| 出版者 | Elsevier B.V. |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/60353]  |
| 作者单位 | 1.School of Chemical Engineering, Zhengzhou University, Zhengzhou; Henan; 450000, China 2.Beijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing; 100190, China 3.Zhongke Langfang Institute of Process Engineering, Langfang; Hebei; 065001, China 4.Zhengzhou Zhongke Emerging Industry Technology Research Institute, Zhengzhou; Henan; 450000, China 5.Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing; 100190, China |
| 推荐引用方式 GB/T 7714 | Yao, Jiahao,Li, Zhuang,Ji, Xiuling,et al. Novel enzyme-metal-organic framework composite for efficient cadaverine production[J]. Biochemical Engineering Journal,2021,176. |
| APA | Yao, Jiahao.,Li, Zhuang.,Ji, Xiuling.,Xue, Yaju.,Ren, Baozeng.,...&Huang, Yuhong.(2021).Novel enzyme-metal-organic framework composite for efficient cadaverine production.Biochemical Engineering Journal,176. |
| MLA | Yao, Jiahao,et al."Novel enzyme-metal-organic framework composite for efficient cadaverine production".Biochemical Engineering Journal 176(2021). |
入库方式： OAI收割
来源：过程工程研究所
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