Overexpression and biochemical characterization of a truncated endo-伪 (1 鈫?3)-fucoidanase from alteromonas sp. SN-1009
文献类型：期刊论文
| 作者 | Zhu, Chenlu3,4; Liu, Zebin1; Ren, Lishi3; Jiao, Siming3; Zhang, Xuebing3; Wang, Qiukuan2; Li, Zhimin4; Du, Yuguang3; Li, Jian-Jun3 |
| 刊名 | Food Chemistry
 |
| 出版日期 | 2021-08-15 |
| 卷号 | 353 |
| 关键词 | Sodium chloride - Degradation |
| ISSN号 | 3088146 |
| DOI | 10.1016/j.foodchem.2021.129460 |
| 英文摘要 | Endo-fucoidanases are important in structural analysis of fucoidans and preparation of fuco-oligosaccharides. However their enzymological properties and analysis of degradation products are scarcely investigated. Truncated endo-伪 (1 鈫?3)-fucoidanase Fda1 (tFda1B from Alteromonas sp. was overexpressed and characterized, showing highest activity at pH 7.0, 35 掳C, and 1.0 M NaCl. Its Km and kcat were 3.88 卤 0.81 mg/mL and 0.82 卤 0.17 min鈭?. Fe3+ and Mn2+ enhanced activity by 100% and 19.5% respectively. Co2+ and Cu2+ completely inactivated tFda1B, whereas Ni2+, Mg2+, Zn2+, Pb2+, Ca2+, Ba2+ and Li+ decreased activity by 58.8%, 56.0%, 50.6%, 47.7%, 28.9%, 15.6% and 37.5%, respectively. Catalytic residues were identified through structure and sequence alignment, and confirmed by mutagenesis. Degradation products of Kjellmaniella crassifolia fucoidan by tFda1B were characterized by LC-ESI-MS/MS, confirming tFda1B belongs to endo-(1 鈫?3)-fucoidanases, and backbone of K. crassifolia fucoidan is 1 鈫?3 fucoside linkage. This endo-伪 (1 鈫?3)-fucoidanase would be useful for elucidating fucoidan structures, and be used as a food enzyme. 漏 2021 Elsevier Ltd |
| 学科主题 | Oligosaccharides |
| 项目编号 | We are grateful to the National Key R&D Program of China (2018YFC0311105) for the financial support. |
| 出版者 | Elsevier Ltd |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/60361]  |
| 作者单位 | 1.College of Life Sciences, Capital Normal University, Beijing; 10048, China 2.National R & D Branch Center for Seaweed Processing, Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, College of Food Science and Engineering, Dalian Ocean University, Dalian; 116023, China 3.National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, Beijing; 100190, China 4.College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang; 330045, China |
| 推荐引用方式 GB/T 7714 | Zhu, Chenlu,Liu, Zebin,Ren, Lishi,等. Overexpression and biochemical characterization of a truncated endo-伪 (1 鈫?3)-fucoidanase from alteromonas sp. SN-1009[J]. Food Chemistry,2021,353. |
| APA | Zhu, Chenlu.,Liu, Zebin.,Ren, Lishi.,Jiao, Siming.,Zhang, Xuebing.,...&Li, Jian-Jun.(2021).Overexpression and biochemical characterization of a truncated endo-伪 (1 鈫?3)-fucoidanase from alteromonas sp. SN-1009.Food Chemistry,353. |
| MLA | Zhu, Chenlu,et al."Overexpression and biochemical characterization of a truncated endo-伪 (1 鈫?3)-fucoidanase from alteromonas sp. SN-1009".Food Chemistry 353(2021). |
入库方式： OAI收割
来源：过程工程研究所
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