Haloalkaliphilic Thioalkalivibrio, a dominant genus for sulfide removal, has attracted growing interest. However, the bacterial biological response to this process's final product, sulfate, has not been well-studied. Here, thiosulfate oxidation and sulfur formation by T. versutus D301 were being enhanced with increasing sulfate supply. With the addition of 0.73 M sulfate, the thiosulfate utilization rate and sulfur production were improved by 68.1% and 120.1% compared with carbonate-grown control at the same salinity (1.8 M). For sulfate-grown cells, based on metabolic analysis, the downregulation of central carbon metabolism indicated that sulfate triggered a decrease in energy conservation efficiency. Additionally, the gene expression analysis further revealed that sulfate induced the inhibition of sulfur to sulfate oxidation, causing the upregulation of thiosulfate to sulfur oxidation for providing cells with additional energy. This study enhances researchers鈥?understanding regarding the sulfate effect on the bio-desulfurization process and presents a new perspective of optimizing the biotechniques. 漏 2021 Elsevier Ltd