Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity
文献类型：期刊论文
| 作者 | Yan, Xing-Ran1,2; Li, Juan2; Na, Xiang-Ming2; Li, Tong2; Xia, Yu-Fei1,2; Zhou, Wei-Qing1,2; Ma, Guang-Hui1,2 |
| 刊名 | Chinese Journal of Polymer Science (English Edition)
 |
| 出版日期 | 2022-09-01 |
| 卷号 | 40期号:9页码:1080-1089 |
| ISSN号 | 2567679 |
| 关键词 | Cell culture - Emulsification - Emulsion polymerization - Emulsions - Flowcharting - Stem cells |
| DOI | 10.1007/s10118-022-2800-7 |
| 英文摘要 | Mesenchymal stem cells (MSCs) have attracted great attention as a source of cells for regenerative medicine owing to their self-renewal ability and multiple differentiation potential. However, the serious shortage of MSCs and the difficulty of maintaining the differentiation potential during culture in vitro limit their application. Microcarrier culture technology is an effective method to realize large-scale culture of MSCs, whereas the effect of microcarriers properties on MSCs proliferation and differentiation potential maintenance should be investigated. In this study, Konjac glucomannan (KGM) microcarriers with a wide range of rigidity from 0.2 MPa to 10 MPa were prepared using water-in-oil emulsion polymerization. It was found that the microcarriers rigidity had great influence on MSCs by regulating cell spreading rate. Rapid proliferation and good differentiation potential maintenance of MSCs were achieved on the intermediate rigidity about 2.51卤0.65 MPa with the faster spreading rate of cells. As a result, MSCs on 2.51卤0.65 MPa KGM microcarriers proliferated about 27 times, which was 1.7 times as much as MSCs cultured on the commercial microcarriers Cytodex-1. The differentiation potential was also improved about 3.2 times compared to Cytodex-1. Therefore, it is indispensable to regulate microcarriers rigidity, especially for application of MSCs. 漏 2022, Chinese Chemical Society Institute of Chemistry, Chinese Academy of Sciences. |
| 学科主题 | Rigidity |
| 项目编号 | This work was financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA16020405), National Key Research and Development Program (2020YFA0112603), the National Natural Science Foundation of China (Nos. 21902160 and 21821005). |
| 出版者 | Springer Verlag |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/61178]  |
| 作者单位 | 1.University of Chinese Academy of Sciences, Beijing; 100049, China 2.State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing; 100190, China |
| 推荐引用方式 GB/T 7714 | Yan, Xing-Ran,Li, Juan,Na, Xiang-Ming,et al. Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity[J]. Chinese Journal of Polymer Science (English Edition),2022,40(9):1080-1089. |
| APA | Yan, Xing-Ran.,Li, Juan.,Na, Xiang-Ming.,Li, Tong.,Xia, Yu-Fei.,...&Ma, Guang-Hui.(2022).Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity.Chinese Journal of Polymer Science (English Edition),40(9),1080-1089. |
| MLA | Yan, Xing-Ran,et al."Mesenchymal Stem Cells Proliferation on Konjac Glucomannan Microcarriers: Effect of Rigidity".Chinese Journal of Polymer Science (English Edition) 40.9(2022):1080-1089. |
入库方式： OAI收割
来源：过程工程研究所
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