Degradation of phenolic inhibitors by laccase immobilized on tannic acid/polyethylenimine modified magnetic nanoparticles
文献类型：期刊论文
| 作者 | Li, Shiqian1,2; Qi, Benkun2; Luo, Jianquan1,2; Wan, Yinhua1,3 |
| 刊名 | Results in Engineering
 |
| 出版日期 | 2022-09-01 |
| 卷号 | 15 |
| 关键词 | Cellulose - Costs - Degradation - Enzymatic hydrolysis - Enzyme activity - Flavonoids - Magnetite - Nanomagnetics - Reusability - Tannins |
| DOI | 10.1016/j.rineng.2022.100585 |
| 英文摘要 | Magnetic Fe3O4 nanoparticles were successfully functionalized with the low-cost mussel-inspired tannic acid (TA)/polyethylenimine (PEI) under mild conditions and then activated with glutaraldehyde (GA) to covalently immobilize laccase for degradation of four model phenolic inhibitors commonly found in lignocellulosic hydrolysate. The immobilized laccase showed the highest relative enzyme activity with the laccase loading of 39.9 mg/g and the enzyme activity recovery of 53.1%. Compared with free laccase, immobilized laccase possessed better relative enzyme activity at acidic pH and wide temperature range, as well as exhibited excellent storage stability and satisfactory reusability. Moreover, it was found that the phenolic degradation products by immobilized laccase did not exhibit obvious inhibition on subsequent enzymatic cellulose hydrolysis. Thus, the low-cost TA/PEI coating for modification of magnetic nanoparticles will provide a promising support for laccase immobilization and the immobilized laccase has great potential in removal of phenolic inhibitors present in lignocellulosic hydrolysate. 漏 2022 The Authors |
| 学科主题 | Enzyme Immobilization |
| 项目编号 | This work was supported by the National Key R&D Program of China (Grant No. 2018YFB1501701 ). |
| 出版者 | Elsevier B.V. |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/61203]  |
| 作者单位 | 1.University of Chinese Academy of Sciences, Beijing; 100049, China 2.State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing; 100190, China 3.Ganjiang Innovation Academy, Chinese Academy of Sciences, Jiangxi, Ganzhou, China |
| 推荐引用方式 GB/T 7714 | Li, Shiqian,Qi, Benkun,Luo, Jianquan,et al. Degradation of phenolic inhibitors by laccase immobilized on tannic acid/polyethylenimine modified magnetic nanoparticles[J]. Results in Engineering,2022,15. |
| APA | Li, Shiqian,Qi, Benkun,Luo, Jianquan,&Wan, Yinhua.(2022).Degradation of phenolic inhibitors by laccase immobilized on tannic acid/polyethylenimine modified magnetic nanoparticles.Results in Engineering,15. |
| MLA | Li, Shiqian,et al."Degradation of phenolic inhibitors by laccase immobilized on tannic acid/polyethylenimine modified magnetic nanoparticles".Results in Engineering 15(2022). |
入库方式： OAI收割
来源：过程工程研究所
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