We present a study of high-resolution spectra of RS Canum Venaticorum (RS CVn), a prototype of active binary systems. Our data were obtained from 1998 to 2017 using different telescopes. We analyse the chromospheric activity indicators IRT, Hα, D1, D2 doublet, D3, and Hβ using a spectral subtraction technique. The chromospheric emission stems mainly from the K2 IV primary star, while the F5 V secondary star only shows weak emission features in a few of our spectra. We find excess absorption features in the subtracted Hα lines and other activity indicators from spectra taken near primary eclipse, which we ascribe to prominence-like material associated with the primary star. We estimate size limits of these tentative prominences based on the geometry of the binary system, and investigate the physical properties of the strongest prominence. An optical flare, characterized by D3 line emission, together with stronger emission in other activity lines, was detected. The flare energy is roughly comparable to strong flares observed on other RS CVn-type stars. The chromospherically active longitudes of RS CVn most frequently appear near the two quadratures of the system and display changes between observing runs, which indicates an ongoing evolution of its active regions. © 2023 The Author(s) Published by Oxford University Press on behalf of Royal Astronomical Society.