Laser tweezers Raman spectroscopy combined with deep learning to classify marine bacteria
文献类型:期刊论文
作者 | Liu, Bo2,4; Liu, Kunxiang2,4; Wang, Nan5; Ta, Kaiwen3; Liang, Peng2,4; Yin, Huabing1; Li, Bei2,4 |
刊名 | TALANTA |
出版日期 | 2022-07-01 |
卷号 | 244页码:6 |
ISSN号 | 0039-9140 |
关键词 | Progressive generative adversarial network Residual network Raman spectroscopy Optical tweezers Classification Deep-sea microorganism |
DOI | 10.1016/j.talanta.2022.123383 |
通讯作者 | Li, Bei |
目次 | 否 |
英文摘要 | Rapid identification of marine microorganisms is critical in marine ecology, and Raman spectroscopy is a promising means to achieve this. Single cell Raman spectra contain the biochemical profile of a cell, which can be used to identify cell phenotype through classification models. However, traditional classification methods require a substantial reference database, which is highly challenging when sampling at difficult-to-access locations. In this scenario, only a few spectra are available to create a taxonomy model, making qualitative analysis difficult. And the accuracy of classification is reduced when the signal-to-noise ratio of a spectrum is low. Here, we describe a novel method for categorizing microorganisms that combines optical tweezers Raman spectroscopy, Progressive Growing of Generative Adversarial Nets (PGGAN), and Residual network (ResNet) analysis. Using the optical Raman tweezers, we acquired single cell Raman spectra from five deep-sea bacterial strains. We randomly selected 300 spectra from each strain as the database for training a PGGAN model. PGGAN generates a large number of high-resolution spectra similar to the real data for the training of the residual neural network. Experimental validations show that the method enhances machine learning classification accuracy while also reducing the demand for a considerable amount of training data, both of which are advantageous for analyzing Raman spectra of low signal-to-noise ratios. A classification model was built with this method, which reduces the spectra collection time to 1/3 without compromising the classification accuracy. |
WOS关键词 | SINGLE ; TRENDS ; CELLS |
资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22020403] ; National Natural Science Foundation of China[42006061] |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000788737800001 |
资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China |
版本 | 出版稿 |
源URL | [http://ir.idsse.ac.cn/handle/183446/9443] |
专题 | 深海科学研究部_深海地质与地球化学研究室 |
通讯作者 | Li, Bei |
作者单位 | 1.Univ Glasgow, James Watt Sch Engn, Glasgow G12 8LT, Lanark, Scotland 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Hainan, Peoples R China 4.Chinese Acad Sci, Changchun Inst Opt Fine Mech & Phys, State Key Lab Appl Opt, Changchun 130033, Peoples R China 5.Hooke Instruments Ltd, Changchun 130033, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Bo,Liu, Kunxiang,Wang, Nan,et al. Laser tweezers Raman spectroscopy combined with deep learning to classify marine bacteria[J]. TALANTA,2022,244:6. |
APA | Liu, Bo.,Liu, Kunxiang.,Wang, Nan.,Ta, Kaiwen.,Liang, Peng.,...&Li, Bei.(2022).Laser tweezers Raman spectroscopy combined with deep learning to classify marine bacteria.TALANTA,244,6. |
MLA | Liu, Bo,et al."Laser tweezers Raman spectroscopy combined with deep learning to classify marine bacteria".TALANTA 244(2022):6. |
入库方式: OAI收割
来源:深海科学与工程研究所
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