Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents
文献类型：期刊论文
| 作者 | Guo LL(郭璐璐) ; Wang SL(王少露); Zhu FC(朱方超); Xue F(薛峰); He LS(贺丽生)
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| 刊名 | Marine drugs
 |
| 出版日期 | 2021 |
| 卷号 | 19期号:11页码:13 |
| 关键词 | crustins antibacterial peptides hydrothermal vent anti-Gram-negative bacteria Al-crus 3 and Al-crus 7 |
| DOI | 10.3390/md19110600 |
| 通讯作者 | He LS(贺丽生) |
| 英文摘要 | Crustins are widely distributed among different crustacean groups. They are characterized by a whey acidic protein (WAP) domain, and most examined Crustins show activity against Gram-positive bacteria. This study reports two Crustins, Al-crus 3 and Al-crus 7, from hydrothermal vent shrimp, Alvinocaris longirostris. Al-crus 3 and Al-crus 7 belong to Crustin Type IIa, with a similarity of about 51% at amino acid level. Antibacterial assays showed that Al-crus 3 mainly displayed activity against Gram-positive bacteria with MIC50 values of 10-25 mu M. However, Al-crus 7 not only displayed activity against Gram-positive bacteria but also against Gram-negative bacteria Imipenem-resistant Acinetobacter baumannii, in a sensitive manner. Notably, in the effective antibacterial spectrum, Methicillin-sensitive Staphylococcus aureus, Escherichia coli (ESBLs) and Imipenem-resistant A. baumannii were drug-resistant pathogens. Narrowing down the sequence to the WAP domain, Al-crusWAP 3 and Al-crusWAP 7 demonstrated antibacterial activities but were weak. Additionally, the effects on bacteria did not significantly change after they were maintained at room temperature for 48 h. This indicated that Al-crus 3 and Al-crus 7 were relatively stable and convenient for transportation. Altogether, this study reported two new Crustins with specific characteristics. In particular, Al-crus 7 inhibited Gram-negative imipenem-resistant A. baumannii. |
| WOS关键词 | BLACK TIGER SHRIMP ; RECOMBINANT EXPRESSION ; ANTIMICROBIAL PEPTIDES ; MOLECULAR-CLONING ; PENAEUS-MONODON ; ANTIBACTERIAL PEPTIDE ; SHORE CRAB ; HEMOCYTES ; INFECTION ; PROTEIN |
| 资助项目 | Institute and Local Government Cooperation Projects of Sanya[2019YD03] ; Major Scientific and Technological Projects of Hainan Province[ZDKJ2019011] ; National Key Research and Development Program of China[2018YFC0309804] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000806913700001 |
| 出版者 | MDPI |
| 资助机构 | Institute and Local Government Cooperation Projects of Sanya ; Major Scientific and Technological Projects of Hainan Province ; National Key Research and Development Program of China |
| 版本 | 出版稿 |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/9046]  |
| 专题 | 深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组 |
| 通讯作者 | He LS(贺丽生) |
| 作者单位 | 1.中国科学院深海科学与工程研究所 2.北京大学 |
| 推荐引用方式 GB/T 7714 | Guo LL,Wang SL,Zhu FC,et al. Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents[J]. Marine drugs,2021,19(11):13. |
| APA | Guo LL,Wang SL,Zhu FC,Xue F,&He LS.(2021).Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents.Marine drugs,19(11),13. |
| MLA | Guo LL,et al."Characteristics of two crustins from Alvinocaris longirostris in Hydrothermal Vents".Marine drugs 19.11(2021):13. |
入库方式： OAI收割
来源：深海科学与工程研究所
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