Application of spectrochemical analysis with chemometrics to profile biochemical alterations in nanoplastic-exposed HepG2 cells
文献类型：期刊论文
| 作者 | Xing, Yu2; Li, Jing4; Yang, Jingjing5; Li, Junyi6; Pang, Weiyi3; Martin, Francis L.1,7; Xu, Li2 |
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2023-11-01 |
| 卷号 | 336页码:12 |
| ISSN号 | 0269-7491 |
| 关键词 | ATR-FTIR spectroscopy Raman spectroscopy Nanoplastics Biochemical alterations |
| DOI | 10.1016/j.envpol.2023.122309 |
| 通讯作者 | Xu, Li(xuliforever@163.com) |
| 英文摘要 | Humans are routinely exposed to nanoplastics (NPs) in various ways, and this exposure presents a significant health risk. Nevertheless, there remain gaps in our knowledge, particularly in the mechanisms of toxicity of NPs with different surface charges at very low environmental concentrations. Herein, a spectrochemical approach was used to profile the cytotoxicity of NPs with different surface charges in HepG2 cells. It was found that all three NPs can cause some biomolecular alterations in cells, affecting cellular lipids, proteins, amino acids, and genetic material. Of these, PS and PS-COOH led to a non-linear dose-response, which may be related to a biphasic dose-response, whereas PS-NH2 led to a linear dose-response with a gradual increase in toxicity with increasing exposure concentration. In addition, the spectroscopic results showed that surface modifications led to cellular biochemical changes and caused adverse biological effects, with PS-NH2 exhibiting higher toxicity compared to PS or PS-COOH along with an inhibition of cell proliferation. Surprisingly PS-COOH, although considered the least toxic NP, appears to cause DNA damage. Overall, the toxic effects of different surface-modified NPs in cells were detected for the first time by applying spectrochemical techniques, and these findings provide important data towards understanding the emerging widespread environmental pollution of NPs and their effects on |
| WOS关键词 | RAMAN-SPECTROSCOPY ; RANDOM COIL ; TOXICITY ; TRANSPORT |
| 资助项目 | Third Xinjiang Scienfic Expedition Program[2022xjkk0901] ; Capacity-building Projects by the Beijing Academy of Agriculture and Forestry Sciences[KJCX20220414] ; Capacity-building Projects by the Beijing Academy of Agriculture and Forestry Sciences[KJCX2023421] |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:001060351100001 |
| 资助机构 | Third Xinjiang Scienfic Expedition Program ; Capacity-building Projects by the Beijing Academy of Agriculture and Forestry Sciences |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/194708]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Xu, Li |
| 作者单位 | 1.Blackpool Teaching Hosp NHS Fdn Trust, Dept Cellular Pathol, Whinney Heys Rd, Blackpool FY3 8NR, England 2.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing Technol, Beijing 100097, Peoples R China 3.Guilin Med Univ, Sch Publ Hlth, Guilin 541199, Peoples R China 4.Chinese Acad Sci, Key Lab Ecosyst Network Observat & Modeling, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China 5.Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resour, Sch Med & Holist Integrat Med, Dept Biochem & Mol Biol, Nanjing 210023, Peoples R China 6.Natl Univ Singapore, Suzhou Res Inst, Suzhou 215125, Peoples R China 7.Biocel UK Ltd, Kingston Upon Hull HU10 7TS, England |
| 推荐引用方式 GB/T 7714 | Xing, Yu,Li, Jing,Yang, Jingjing,et al. Application of spectrochemical analysis with chemometrics to profile biochemical alterations in nanoplastic-exposed HepG2 cells[J]. ENVIRONMENTAL POLLUTION,2023,336:12. |
| APA | Xing, Yu.,Li, Jing.,Yang, Jingjing.,Li, Junyi.,Pang, Weiyi.,...&Xu, Li.(2023).Application of spectrochemical analysis with chemometrics to profile biochemical alterations in nanoplastic-exposed HepG2 cells.ENVIRONMENTAL POLLUTION,336,12. |
| MLA | Xing, Yu,et al."Application of spectrochemical analysis with chemometrics to profile biochemical alterations in nanoplastic-exposed HepG2 cells".ENVIRONMENTAL POLLUTION 336(2023):12. |
入库方式： OAI收割
来源：地理科学与资源研究所
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